Anti-Mouse CD8a Monoclonal Antibody () for Flow Cytometry - P01731

Core Product Specifications and Parameters

Biological Background: CD8a Function and Localization

• CD8a, also known as Lyt-2, is a T-cell surface glycoprotein and a member of the immunoglobulin superfamily.
• It functions as a coreceptor for MHC class I molecules, interacting simultaneously with the T-cell receptor and MHC class I on antigen-presenting cells.
• Upon antigen recognition, CD8a recruits the Src kinase LCK to the TCR-CD3 complex, initiating intracellular signaling for T-cell activation, cytokine production, and cytotoxic responses.
• In cytotoxic T lymphocytes (CTLs), this mechanism enables the recognition and elimination of infected or tumor cells.
• In NK cells, CD8A homodimers promote survival and serial killing of target cells, and support differentiation into memory CD8 T cells.
• The protein is localized at the cell membrane with a single-pass transmembrane domain and contains an extracellular immunoglobulin-like domain.
• Key post-translational modifications include glycosylation, disulfide bond formation, and palmitoylation, which are important for its stability and function.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (amino acids 28-196), which overlaps with the MHC class I binding region; thus, the antibody likely recognizes the native surface protein. Consider validating binding on live cells by FC.
• For flow cytometry, optimal antibody concentration should be titrated on mouse lymphocytes (e.g., splenocytes or thymocytes) as a positive control. Use appropriate isotype control.
• Because CD8a is a transmembrane protein, fixation and permeabilization steps are typically not required for surface staining, but ensure cells are kept viable during processing.
• If using in combination with other fluorophore-conjugated antibodies, check for potential spectral overlap with (similar to FITC).

CamelBio: Your One-Stop Sourcing Bridge

• CamelBio provides diagnostic manufacturers, laboratories, and research institutes with comprehensive access to IVD raw materials, including validated monoclonal antibodies like this anti-CD8a clone. We support your immune-oncology and T-cell research workflows with reliable bulk reagents and ancillary products.
• Beyond standard catalog antibodies, CamelBio can source specialized raw materials—such as antibody pairs, optimized conjugates, and rare target reagents—to streamline your assay development from concept to clinic.