Anti-Mouse CD8a Monoclonal Antibody for FC - P01731

Core Product Specifications and Parameters

Biological Background: CD8 alpha chain Function and Localization

• Identified as T-cell surface glycoprotein CD8 alpha chain (also known as Lyt-2), an integral membrane glycoprotein of the immunoglobulin superfamily.
• In T-cells, functions as a co-receptor for MHC class I:peptide complexes, where it simultaneously binds the TCR and MHC I on antigen-presenting cells, recruiting the Src kinase LCK to initiate signaling cascades that lead to cytotoxic T-lymphocyte (CTL) activation, lymphokine production, motility, and adhesion.
• The MHC class I pathway presents peptides derived from cytosolic proteins, enabling CTLs to recognize and eliminate infected or tumor cells.
• In NK-cells, CD8A homodimers provide a survival mechanism that promotes conjugation and lysis of multiple target cells, and facilitates differentiation of activated lymphocytes into memory CD8 T-cells.
• Subcellular localization is primarily at the cell membrane, consistent with its role in antigen recognition and intercellular signaling.
• Post-translational modifications include glycosylation, disulfide bond formation, and palmitoylation, which contribute to its stability and membrane association.
• Expressed on cytotoxic T lymphocytes and a subset of natural killer cells, making it a key marker for these immune cell populations in mouse.

Experimental Guidance and Technical Tips

• This antibody is a rabbit monoclonal conjugated to , allowing direct detection without a secondary reagent; consider titrating to determine the optimal concentration for your specific flow cytometry panel.
• Designed for flow cytometry (FC), it can be used to immunophenotype mouse CD8+ T cells and CD8+ NK cells in splenocyte, lymph node, or peripheral blood preparations.
• As it is validated for mouse reactivity only, ensure species compatibility for your experimental model; cross-reactivity with other species has not been tested.
• Include appropriate isotype controls and viability dyes to minimize background and non-specific binding.
• The immunogen (amino acids 28-196 of the extracellular domain) targets a region likely to be accessible on the cell surface; confirm expression levels in your cell population of interest.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive IVD raw-material sourcing solutions for diagnostic developers and research laboratories. This anti-mouse CD8a monoclonal antibody supports immunology and immuno-oncology research, and we can supply validated antibody pairs, bulk ancillary reagents, and optimized monoclonal antibodies to accelerate your assay development. Whether you need established products like this or assistance sourcing rare-target raw materials, CamelBio bridges every step from concept to clinic.