Anti-Mouse CD8a Monoclonal Antibody for FC - P01731

Core Product Specifications and Parameters

Biological Background: CD8a Function and Localization

• CD8a (T-cell surface glycoprotein CD8 alpha chain, also known as Lyt-2) is an integral membrane glycoprotein that serves as a coreceptor for MHC class I molecules on T-cells, enabling cytotoxic T-lymphocyte (CTL) activation and target cell killing.
• The protein interacts simultaneously with the T-cell receptor (TCR) and MHC class I, recruiting the Src kinase LCK to the TCR-CD3 complex, which initiates intracellular signaling pathways leading to lymphokine production, motility, and adhesion.
• In natural killer (NK) cells, CD8A homodimers provide a survival mechanism, promoting conjugation and lysis of multiple target cells and supporting the differentiation of activated lymphocytes into memory CD8 T-cells.
• The protein is localized to the cell membrane and undergoes alternative splicing, producing isoforms with distinct functional properties.
• Post-translational modifications include disulfide bond formation, glycosylation, and palmitoylation, which regulate its structure and membrane anchoring.
• CD8a is a member of the immunoglobulin superfamily and plays a critical role in adaptive immunity against intracellular pathogens and tumor surveillance.

Experimental Guidance and Technical Tips

• The immunogen comprises amino acids 28-196 of mouse CD8a, covering the extracellular domain. When designing flow cytometry panels, consider that this antibody may recognize both CD8αα homodimers and CD8αβ heterodimers if the epitope is accessible.
• For optimal performance in flow cytometry, titrate the antibody to determine the optimal staining concentration for your cell type of interest. Include appropriate isotype controls and validate in the relevant mouse tissue or cell line system.
• If using for intracellular staining, note that the epitope is in the extracellular domain; permeabilization may not be necessary but could affect signal.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research labs with reliable access to IVD-grade raw materials. This anti-CD8a monoclonal antibody is ideal for developing T-cell immunophenotyping assays and immune monitoring panels. Beyond antibodies, CamelBio offers validated antibody pairs, optimized monoclonal and polyclonal reagents, and bulk ancillary materials to streamline your assay development and production.