Anti-Mouse CD84 Monoclonal Antibody () for Flow Cytometry - Q18PI6

Core Product Specifications and Parameters

Biological Background: CD84 (SLAMF5) Function and Localization

• CD84 is a self-ligand receptor of the signaling lymphocytic activation molecule (SLAM) family, modulating activation and differentiation of immune cells and bridging innate and adaptive immunity. Related references: PMID:20962259
• CD84 can mediate natural killer (NK) cell cytotoxicity, dependent on the adapter proteins SH2D1A and SH2D1B. Related references: PMID:20962259
• CD84 enhances T-cell proliferative responses independently of SH2D1A.
• CD84 is required for prolonged T-cell:B-cell contact and optimal T follicular helper function, ensuring germinal center formation. Related references: PMID:20153220
• In germinal centers, CD84 maintains B cell tolerance and prevents autoimmunity. Related references: PMID:25801429
• In macrophages, CD84 enhances LPS-induced MAPK and NF-kappaB activation, modulating cytokine secretion. Related references: PMID:20628063
• CD84 is located on the cell membrane. It is predominantly expressed in hematopoietic tissues, including lymph node, spleen, thymus, and bone marrow, and is also detected in lung.
• CD84 is a glycoprotein and phosphoprotein with immunoglobulin domains and disulfide bonds; it undergoes alternative splicing.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (aa 22-221), making this antibody suitable for detecting CD84 on live cells by flow cytometry.
• Optimize antibody titration for your specific cell type and staining conditions to achieve the best signal-to-noise ratio.
• Include appropriate isotype controls and consider blocking with recombinant CD84 to confirm specificity.
• CD84 expression may be upregulated upon immune cell activation; consider stimulating cells prior to staining if needed.
• The conjugate allows direct detection without a secondary antibody, minimizing non-specific binding.
• Store at 2-8°C, protected from light, and avoid repeated freeze-thaw cycles.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. We supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to accelerate your diagnostic development. For researchers focusing on SLAM family proteins like CD84, involved in immune regulation and autoimmune diseases, CamelBio offers tailored supply solutions and dedicated support. Contact us to discuss your specific requirements.