Anti-Mouse CD82 Monoclonal Antibody for Flow Cytometry - P40237

Core Product Specifications and Parameters

Biological Background: CD82 Function and Localization

• CD82 (Kai1, C33, IA4, Tspan27) is a mouse tetraspanin family metastasis suppressor, functioning as a structural component of tetraspanin-enriched microdomains (TERMs) to regulate cell adhesion, migration, and signaling.
• Attenuates EGF signaling by facilitating ligand-induced EGFR endocytosis and desensitization via PKC-mediated CBL phosphorylation and ubiquitination (By similarity).
• Modulates integrin-mediated cell-matrix adhesion and suppresses migration by organizing membrane integrins such as ITGA4, ITGA6, and ITGB1 (By similarity).
• Participates in immune functions: associates with CD4/CD8 to deliver costimulatory signals, restrains phagocyte migration, and promotes anti-inflammatory macrophage activation. Related references: PMID:35754722
• Facilitates TLR9 trafficking to acidified compartments and myddosome assembly, and inhibits LPS-induced TLR4 signaling. Related references: PMID:31408613
• Recruited to pathogen-containing phagosomes prior to lysosomal fusion and participates in antigen presentation. Related references: PMID:21149584
• Controls angiogenesis by binding and sequestering VEGFA and PDGFA, inhibiting their receptor signaling. Related references: PMID:34530889
• Localizes to the cell membrane, cytoplasmic vesicles, and phagosomes, and is expressed at highest levels in spleen and kidney, with low expression in heart and skeletal muscle.

Experimental Guidance and Technical Tips

• This -conjugated monoclonal antibody is designed for flow cytometry; optimize antibody titration for your specific cell type and experimental conditions.
• CD82 is highly expressed in mouse spleen and kidney; consider using these tissues as positive controls for validation.
• The immunogen encompasses amino acids 1-266 of mouse CD82, corresponding to the large extracellular loop; the antibody may recognize conformational epitopes.
• For intracellular staining, permeabilization and fixation protocols should be validated as CD82 localizes to cytoplasmic vesicles and phagosomes.
• Pair with appropriate isotype controls and viability dyes to ensure accurate population gating in multicolor flow cytometry panels.

CamelBio: Your One-Stop Sourcing Bridge

At CamelBio, we serve as a one-stop sourcing bridge for IVD raw materials, supporting diagnostic manufacturers and research laboratories with comprehensive access to monoclonal antibodies, recombinant proteins, and ancillary reagents. Our CD82 antibody offering addresses key research areas in cancer metastasis, immunology, and cell signaling. Beyond individual antibodies, CamelBio supplies validated antibody pairs, bulk reagents, and custom sourcing for rare targets to accelerate your assay development from concept to clinic.