Anti-Mouse CD80 Monoclonal Antibody () for FC - Q00609

Core Product Specifications and Parameters

Biological Background: CD80 Function and Localization

• CD80 (B7-1) is a costimulatory molecule of the immunoglobulin superfamily that plays a critical role in T-lymphocyte activation by providing the primary auxiliary signal through CD28 receptor binding, leading to NF-kappa-B and MAPK signaling and cytokine production (PubMed:16339518, PubMed:1714935). Related references: PMID:16339518 PMID:1714935
• CD80 also acts as an inhibitor of T-cell activation by engaging CTLA4, a decoy receptor that blocks CD28-mediated T-cell priming, thereby modulating immune responses.
• Expressed on B-cells, CD80 is essential for regulating B-cell and T-cell interactions during both early and late germinal center responses, crucial for effective humoral immunity (PubMed:22450810). Related references: PMID:22450810
• Upon CD28 engagement, CD80 stimulates T-cell metabolic reprogramming by enhancing glucose metabolism and ATP synthesis via the PI3K/Akt pathway.
• The Cd80 gene encodes a transmembrane glycoprotein of approximately 34.6 kDa, featuring an immunoglobulin domain, a transmembrane helix, disulfide bond, and alternative splicing isoforms.
• CD80 localizes to the cell membrane and is predominantly expressed on activated B-cells, gamma interferon-stimulated monocytes, and certain B-cell malignancies.
• Key post-translational modifications include glycosylation and a signal peptide that directs proper membrane localization.

Experimental Guidance and Technical Tips

• The antibody is directly conjugated to , allowing immediate use in flow cytometry without secondary detection steps.
• Titrate the antibody over a range (e.g., 0.1–10 µg/mL) to determine optimal staining concentration for your specific cell type and experimental conditions.
• The immunogen spans the extracellular domain (amino acids 38–245), suggesting recognition of the native surface protein; verify performance with fixed or permeabilized samples if required.
• Include appropriate isotype controls (Armenian hamster IgG) and live/dead discrimination to ensure specific gating.
• This antibody is reported to react specifically with mouse CD80; cross-reactivity with other species is not expected but should be validated if needed.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated IVD raw-material partner, CamelBio connects diagnostic manufacturers and research labs with high-quality antibodies like this anti-mouse CD80 clone, supporting immuno-oncology and immune profiling workflows. Beyond individual reagents, we supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and offer sourcing assistance for rare targets—streamlining your path from concept to clinic.