Anti-Mouse CD79b Monoclonal Antibody () for Flow Cytometry - P15530

Core Product Specifications and Parameters

Biological Background: CD79b (Ig-beta) Function and Localization

• CD79b is the beta chain (Ig-beta / B29) of the B-cell antigen receptor (BCR) complex, essential for B-cell development and function.
• It cooperates with CD79a (Ig-alpha) to initiate signal transduction upon antigen binding, leading to BCR internalization and trafficking to late endosomes for antigen presentation.
• CD79b enhances phosphorylation of CD79a, likely by recruiting kinases or proteins that protect CD79a from dephosphorylation, thereby amplifying BCR signaling.
• The protein is expressed on the cell membrane of B lymphocytes and serves as a pan-B-cell marker.
• It contains an extracellular immunoglobulin-like domain, a single transmembrane helix, and a cytoplasmic tail with immunoreceptor tyrosine-based activation motifs (ITAMs) involved in downstream signaling.
• Post-translational modifications include phosphorylation, glycosylation, disulfide bond formation, and ubiquitination.
• The full-length precursor has a predicted mass of ~25.7 kDa; the mature protein (residues 26–228) runs at approximately 25 kDa in SDS-PAGE, often with some glycosylation.

Experimental Guidance and Technical Tips

• This -conjugated antibody allows direct detection of CD79b on live cells without secondary reagents, minimizing background and simplifying the flow cytometry workflow.
• For optimal staining, titrate the antibody on mouse B-cell lines or primary murine splenocytes to determine the best concentration for your specific application.
• Include an appropriate isotype control and a viability dye to exclude dead cells, which can cause non-specific binding.
• CD79b is a surface marker; staining can typically be performed without fixation or permeabilization, but verify protocols for your experimental system.
• Consider using this antibody in multi-color panels with other B-cell markers (e.g., CD19, CD45R/B220) for detailed immunophenotyping.

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