Anti-Mouse CD79b Monoclonal Antibody for Flow Cytometry - P15530

Core Product Specifications and Parameters

Biological Background: CD79b Function and Localization

• CD79b is the beta chain of the B-cell antigen receptor complex, also known as B29, Ig-beta, or immunoglobulin-associated B29 protein.
• Forms a heterodimer with CD79a (Ig-alpha) essential for BCR signal transduction, leading to antigen internalization, trafficking, and presentation.
• Enhances phosphorylation of CD79a, likely by recruiting kinases or protecting it from dephosphorylation.
• Localizes to the cell membrane in B-cells.
• Tissue specificity: expressed specifically in B-cells.
• Contains an immunoglobulin domain and is subject to phosphorylation, glycosylation, and disulfide bond formation.
• Implicated in adaptive immunity and B-cell development; a key marker in immunophenotyping.

Experimental Guidance and Technical Tips

• Conjugated to , a far-red fluorophore, this antibody is optimized for flow cytometry (FC) applications.
• The immunogen spans the extracellular domain (amino acids 26-158), enabling detection of surface CD79b on live cells.
• For best results, titrate the antibody for your specific cell type and always include appropriate isotype controls.
• Validate reactivity in mouse B-cell lines (e.g., splenocytes) or primary B-cells; cross-reactivity is reported only for mouse.
• Note: Protein weight is approximately 25 kDa, corresponding to the glycosylated form in B-cells.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering validated monoclonal antibodies, antibody pairs, and ancillary reagents to accelerate diagnostic development. With a focus on B-cell markers like CD79b, we support research and clinical applications from concept to clinic. Contact us for bulk quantities, custom conjugations, or rare target sourcing.