Anti-Mouse CD69 Monoclonal Antibody () for Flow Cytometry - P37217

Core Product Specifications and Parameters

Biological Background: CD69 Function and Localization

• CD69 (Early activation antigen CD69), also known as AIM and VEA, is a transmembrane protein belonging to the C-type lectin family, functioning as a receptor and signal transducer on the cell surface.
• It is rapidly upregulated on the surface of activated T lymphocytes, B cells, natural killer cells, neutrophils, platelets, and eosinophils upon stimulation, playing a central role in immune cell homeostasis.
• CD69 negatively regulates Th17 cell differentiation via carbohydrate-dependent interaction with galectin-1 on immature dendritic cells, and through the JAK3/STAT5 signaling pathway controlling RORγt transcription.
  Related references: PMID:20696842
• It acts as an oxidized low-density lipoprotein (oxLDL) receptor in CD4 T lymphocytes, negatively regulating the inflammatory response by inducing PDCD1 expression via NFAT activation.
• In eosinophils, CD69 induces IL-10 production through the ERK1/2 pathway, contributing to anti-inflammatory responses.
  Related references: PMID:38540778
• CD69 negatively regulates chemotactic responses of effector lymphocytes and dendritic cells by acting as an S1PR1 agonist, facilitating receptor internalization and degradation.
  Related references: PMID:16525420
• CD69 localizes to the cell membrane and is primarily expressed on activated T-cells, B-cells, natural killer cells, neutrophils, platelets, and eosinophils, with higher abundance in mucosal T-cells.
• Post-translational modifications include glycosylation, phosphorylation, and disulfide bond formation, consistent with its receptor and signaling functions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (amino acids 64-199) of mouse CD69, ensuring specificity for the native protein on the cell surface. Consider this region's conservation when assessing cross-reactivity.
• As an directly conjugated antibody, it is optimized for flow cytometry without the need for secondary detection reagents. Validate staining conditions (antibody concentration, incubation time and temperature) in your specific experimental setup.
• For best results, use fresh or properly cryopreserved mouse cell samples and include appropriate controls (isotype control, viability dye, and Fc receptor blocking) to ensure accurate identification of CD69+ populations.
• The antibody is expected to recognize all activating immune cell types expressing CD69; verify expression kinetics upon stimulation in your model system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw material sourcing, offering a comprehensive portfolio of validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. This anti-mouse CD69 monoclonal antibody supports flow cytometry-based immunology and inflammation research. Beyond catalog products, we specialize in sourcing rare-target raw materials and providing technical consulting to accelerate your diagnostic development from concept to clinic.