Anti-Mouse CD6 Monoclonal Antibody () for Flow Cytometry - Q61003

Core Product Specifications and Parameters

Biological Background: CD6 Function and Localization

• CD6 is a cell adhesion molecule that mediates cell-cell contacts through its interaction with ALCAM/CD166.
• It contributes to signaling cascades triggered by activation of the TCR/CD3 complex, promoting T-cell activation (PubMed:24584089). Related references: PMID:24584089
• Functions as a costimulatory molecule, enhancing T-cell proliferation and contributing to immunological synapse formation and maturation.
• Acts as a calcium-dependent pattern receptor binding Gram-positive and Gram-negative bacteria, LPS, and lipoteichoic acid, leading to MAP kinase activation and pro-inflammatory cytokine secretion.
• Subcellular localization: Cell membrane; single-pass type I membrane protein.
• Tissue specificity: Predominantly expressed in thymus, lymph node, and spleen.
• Post-translational modifications: Glycoprotein, phosphoprotein, contains disulfide bonds; alternative splicing produces multiple isoforms (observed at 64, 68, 72 kDa).

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular region (amino acids 20–155) of mouse CD6, making this antibody suitable for detecting surface expression by flow cytometry.
• As a directly conjugated monoclonal antibody (), it can be used without a secondary antibody; titrate to determine the optimal concentration for your experimental system.
• Include appropriate controls such as isotype controls and CD6 knockout or knockdown validation samples to confirm specificity.
• CD6 is expressed on T cells; ensure your cell preparation and staining protocol preserves surface epitopes.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and biomedical researchers by providing streamlined access to IVD raw materials, including validated antibody pairs and bulk monoclonal antibodies. This anti-CD6 antibody supports T-cell biology and flow cytometry applications, backed by our comprehensive sourcing network. From concept to clinic, CamelBio is your trusted partner for rare-target raw materials, ancillary reagents, and expert technical consultation.