Anti-Mouse CD59a Monoclonal Antibody () for Flow Cytometry - O55186

Core Product Specifications and Parameters

Biological Background: CD59a Function and Localization

• CD59a glycoprotein (also known as protectin, MAC-IP, MACIF) is a potent inhibitor of the complement membrane attack complex (MAC), protecting self-cells from complement-mediated lysis.
• It binds to the C8 and C9 components of the assembling MAC, preventing the incorporation of multiple C9 molecules required for complete pore formation.
• Subcellular location: CD59a is primarily located on the cell membrane, attached via a glycosylphosphatidylinositol (GPI) anchor. A secreted form is also found.
• Tissue specificity: Widely expressed in tissues including liver, kidney, spleen, thymus, brain, and heart, as well as on mononuclear cells, erythrocytes, and platelets. Expression is low in thymus and barely detectable in neutrophils.
• Structural features: The protein contains disulfide bonds, glycosylation sites, and a GPI-anchor. It also includes a signal peptide for secretion.
• Keywords from UniProt: Cell membrane, Secreted, Glycoprotein, GPI-anchor, Disulfide bond, Lipoprotein, Membrane, Reference proteome, Signal.

Experimental Guidance and Technical Tips

• The immunogen covers the extracellular domain (aa 24-96), making the antibody suitable for detecting native CD59a on live cells by flow cytometry.
• For flow cytometry, titrate the antibody to determine the optimal staining concentration in your experimental system.
• Include appropriate controls such as unstained cells and isotype-matched control antibodies to establish gating thresholds.
• Because CD59a is GPI-anchored, consider that treatment with phosphatidylinositol-specific phospholipase C (PI-PLC) may release CD59a from the cell surface, which can be used as a specificity control.
• Validate antibody binding in the relevant cell types or tissue samples, as expression levels may vary (e.g., low in thymus and neutrophils).

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, research laboratories, and institutes with a complete range of IVD raw materials and technical services, from concept to clinic. For complement regulation targets like CD59a, we supply validated monoclonal antibodies and offer bulk reagent sourcing and customization to accelerate your assay development. Our streamlined procurement solutions help you secure high-quality reagents for every stage of your diagnostic project.