Anti-Mouse CD5 Monoclonal Antibody (APC) for Flow Cytometry - P13379

Core Product Specifications and Parameters

Biological Background: CD5 Function and Localization

• CD5, also known as Lymphocyte antigen 1 (Ly-1/Lyt-1), is a lymphoid-specific transmembrane glycoprotein receptor expressed on all T-cells and the B1a subset of B-cells.
• It plays a critical role in regulating TCR and BCR signaling, thymocyte selection, T-cell effector differentiation, and immune tolerance.
  Related references:
  PMID:35720357
• CD5 mediates T-dependent B-cell activation by interacting with B-cell ligands such as CD5L or CD72, and contributes to regulatory T and B-cell homeostasis.
  Related references:
  PMID:24950378, PMID:9064341, PMID:9723705
• It functions as a negative regulator of TCR signaling during thymocyte development by recruiting signaling proteins including LCK, CD3Z chain, PI3K, and CBL.
  Related references:
  PMID:11157848
• CD5 is a single-pass type I membrane protein localized at the cell surface.
• The protein features a signal peptide, an extracellular region with disulfide bonds and N-linked glycosylation sites, and a cytoplasmic tail subject to phosphorylation, consistent with its observed molecular weight of ~54 kDa.

Experimental Guidance and Technical Tips

• This APC-conjugated monoclonal antibody is designed for flow cytometry, targeting the extracellular domain (aa 25-371) of mouse CD5. It is suitable for surface staining of live cells without permeabilization.
• For optimal resolution, titrate the antibody concentration using a known positive control (e.g., mouse splenocytes or thymocytes) to determine the best staining index for your experimental setup.
• Since CD5 is expressed on all T-cells and B1a B-cells, include appropriate lineage markers (e.g., CD3, CD19) to distinguish T-cell and B-cell subsets in multi-color panels.
• Avoid using other APC-conjugated antibodies in the same panel to prevent spectral overlap; consider using PE or FITC conjugates for co-staining.
• Validate staining specificity by including an isotype control or by using CD5-knockout cells if available.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw-material sourcing partner, supplying diagnostic manufacturers and researchers with high-quality antibodies, antigen proteins, and ancillary reagents. For T-cell and B1a cell studies targeting CD5, we provide validated monoclonal antibodies and support bulk procurement, ensuring consistent performance in flow cytometry and beyond. Our portfolio also facilitates rare-target material sourcing and technical consultation to accelerate immunoassay development.