Anti-Mouse CD45 Monoclonal Antibody () for IF/ICC, FC - P06800

Core Product Specifications and Parameters

Biological Background: CD45 Function and Localization

• CD45, also known as leukocyte common antigen (L-CA), Ly-5, and T200, is a receptor-type tyrosine-protein phosphatase C encoded by the Ptprc gene in mouse.
• It is essential for T-cell activation; upon antigen receptor engagement, CD45 positively regulates coactivation via DPP4 binding.
• The protein contains two tandem intracellular phosphatase domains; the first domain is catalytically active, while the second modulates substrate specificity (By similarity).
• CD45 dephosphorylates key signaling molecules including SKAP1, FYN, and LYN, thereby modulating LYN activity and downstream TCR signaling.
• At the immunological synapse, CD45 localizes to membrane rafts and interacts with CLEC10A on dendritic cells, fine-tuning T-cell activation thresholds to prevent autoimmunity.
• Multiple CD45 isoforms exist due to alternative splicing, with molecular weights ranging from 130 to 144 kDa; the protein is heavily glycosylated and phosphorylated.

Experimental Guidance and Technical Tips

• For immunofluorescence (IF/ICC): Fixation and permeabilization conditions may affect epitope accessibility; consider testing methanol versus paraformaldehyde fixation protocols.
• The immunogen spans a large portion of the extracellular domain (aa 26–566), suggesting potential recognition of multiple CD45 isoforms; validate isoform specificity in your experimental context if required.
• For flow cytometry (FC): Titrate the antibody to determine optimal staining concentration, and always include appropriate isotype controls.
• CD45 is broadly expressed on all leukocytes; therefore, expect widespread staining in lymphoid tissues, and use lineage-specific markers for precise cell population gating.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and researchers with one-stop access to premium IVD raw materials, including validated antibody pairs, monoclonal and polyclonal antibodies, and ancillary reagents. For CD45-targeted immunoassays, we supply reliable anti-CD45 antibodies suitable for IF/ICC and flow cytometry, supporting immunology and immuno-oncology assay development. Leverage our sourcing expertise to streamline your reagent procurement from concept to clinical application.