Anti-Mouse CD45 Monoclonal Antibody () for Flow Cytometry - P06800

Core Product Specifications and Parameters

Biological Background: CD45 Function and Localization

• CD45 (Receptor-type tyrosine-protein phosphatase C) is a transmembrane glycoprotein also known as Leukocyte Common Antigen (L-CA), Ly-5, T200, and B220 (specific isoform expressed on B cells). Multiple isoforms are generated by alternative splicing (reflected in Protein Weights: 130, 140, 144 kDa).
• It functions as a protein tyrosine phosphatase essential for T-cell activation through the T-cell receptor, positively regulating co-activation upon binding to DPP4.
• The phosphatase activity resides primarily in the first intracellular PTPase domain; the second domain modulates substrate specificity. Key substrates include SKAP1, FYN, and LYN, modulating lymphocyte signaling.
• CD45 is localized to the cell membrane, enriched in membrane rafts and at the immunological synapse, facilitating dynamic interactions during antigen presentation.
• In addition to T cells, CD45 plays a critical role in B cells (e.g., B220 isoform) and is a pan‑leukocyte marker used for immune cell identification.
• The extracellular domain undergoes extensive glycosylation, which is important for ligand interactions and regulation of phosphatase activity.
• CD45 interacts with CLEC10A on dendritic cells via Tn antigen recognition, modulating T‑cell activation thresholds to prevent autoreactivity.

Experimental Guidance and Technical Tips

• The immunogen comprises amino acids 26–566 of the extracellular domain; thus, the antibody likely binds an extracellular epitope, suitable for live‑cell flow cytometry.
• For FC, we recommend titrating the antibody to determine an optimal concentration for your specific mouse leukocyte population. A starting range of 0.5–2 µg per million cells is a common exploratory window.
• CD45 is a pan‑leukocyte marker; combine with lineage‑specific antibodies (e.g., CD3, CD19) for multicolor immunophenotyping panels.
• The multiple molecular weight forms (130–144 kDa) arise from alternative splicing; validate detection in your model system using appropriate controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and researchers with reliable access to high‑quality IVD raw materials. This anti‑mouse CD45 monoclonal antibody illustrates our capability to supply validated antibodies for flow‑based applications. In addition to bulk monoclonal antibodies, we offer validated antibody pairs, optimized antibody formats, and ancillary reagents to streamline your immunoassay development—all backed by technical and supply‑chain expertise from concept to clinic.