Anti-Mouse CD4 Monoclonal Antibody (PE/Cyanine5) for FC - P06332

Core Product Specifications and Parameters

Biological Background: T-cell surface glycoprotein CD4 Function and Localization

• T-cell surface glycoprotein CD4 (also known as L3T4 or Ly-4) is an integral membrane glycoprotein that serves as a coreceptor for MHC class II molecules, playing a critical role in T-cell activation and immune response.
• CD4 interacts simultaneously with the T-cell receptor (TCR) and MHC class II on antigen-presenting cells, recruiting the Src kinase LCK to the TCR-CD3 complex to initiate intracellular signaling cascades leading to lymphokine production, motility, adhesion, and T-helper cell activation.
• Beyond T-cells, CD4 is expressed in other immune cells such as macrophages, dendritic cells, and NK cells, where it participates in TCR/LCK-independent pathways regulating differentiation, activation, cytokine expression, and cell migration.
• CD4 is essential for T-helper cell development in the thymus and can trigger monocyte differentiation into functional mature macrophages.
• The protein is localized to the cell membrane and features extracellular immunoglobulin-like domains, a single transmembrane helix, and a cytoplasmic tail that undergoes post-translational modifications including phosphorylation, palmitoylation, and N-linked glycosylation.
• CD4 expression is a hallmark of T-helper cells, which are specialized in activating cytotoxic T-cells, regulating B cells, and stimulating phagocytes.

Experimental Guidance and Technical Tips

• For flow cytometry (FC) applications using this PE/Cyanine5 conjugate, titrate the antibody to determine an optimal concentration for your specific cell type and experimental setup to minimize nonspecific binding while retaining sensitivity.
• The immunogen corresponds to the extracellular domain (aa 27–394) of mouse CD4; thus, the antibody is expected to bind the native cell-surface form, but users should validate reactivity in their sample system, especially when working with non-T-cell populations that may express lower CD4 levels.
• Protect the antibody from light and avoid repeated freeze-thaw cycles to maintain conjugate stability and fluorescence intensity.
• Consider using appropriate isotype controls and Fc receptor blocking steps to reduce background in mouse samples.

CamelBio: Your One-Stop Sourcing Bridge

• CamelBio streamlines IVD raw-material procurement for diagnostic developers and researchers by providing validated antibody pairs, optimized recombinant proteins, and ancillary reagents—with this anti-mouse CD4 antibody as an example of our high-quality flow cytometry offerings for immune phenotyping studies.
• From concept to clinic, we support projects requiring rare target sourcing, bulk custom antibody production, and technical consultation, ensuring your CD4-based assays or biomarker panels are backed by reliable supply chains and expert application guidance.