Anti-Mouse CD4 Monoclonal Antibody for Flow Cytometry - P06332

Core Product Specifications and Parameters

Biological Background: CD4 Function and Localization

• CD4 is a T-cell surface glycoprotein belonging to the immunoglobulin superfamily, also designated as L3T4 or Ly-4 in mice. It functions primarily as a coreceptor during T-cell activation.
• During antigen presentation, CD4 interacts with the T-cell receptor (TCR) and binds to the MHC class II molecule on antigen-presenting cells. This interaction recruits the Src kinase LCK, which phosphorylates downstream signaling proteins, leading to T-helper cell activation, lymphokine production, and adhesion.
• CD4 is essential for the development of T-helper cells in the thymus and also promotes the differentiation of monocytes into mature macrophages.
• The expression of CD4 is a hallmark of T-helper cells but is also detected in other immune cells such as macrophages, dendritic cells, and natural killer cells.
• CD4 is an integral membrane protein with its functional domains exposed on the cell surface, consistent with its role in cell–cell interactions and signaling.
• CD4 is a glycoprotein that undergoes palmitoylation and phosphorylation. It contains disulfide bonds and immunoglobulin-like domains, which are critical for structural integrity and interaction with MHC class II molecules. Alternative splicing generates additional isoforms.

Experimental Guidance and Technical Tips

• The conjugate enables direct flow cytometric detection, eliminating the need for secondary staining. Titrate the antibody under your specific experimental conditions to determine the optimal concentration.
• Include appropriate isotype controls and fluorescence minus one (FMO) controls when establishing multicolor panels.
• Although validated for mouse CD4, verify cross-reactivity with other species if required, as only mouse reactivity is guaranteed.
• The immunogen spans the extracellular domain (amino acids 27–394); thus, the antibody likely recognizes a native surface-exposed epitope.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your comprehensive one-stop partner for IVD raw materials, supporting diagnostic manufacturers and research laboratories with a complete portfolio from concept to clinic. Our CD4 antibody products, including validated antibody pairs and optimized monoclonal antibodies, empower T-cell immunology and flow cytometry-based applications. Beyond this antibody, CamelBio excels in sourcing bulk ancillary reagents and rare-target raw materials, ensuring streamlined procurement for your diagnostic and research programs.