Anti-Mouse CD4 Monoclonal Antibody for Flow Cytometry - P06332

Core Product Specifications and Parameters

Biological Background: CD4 Function and Localization

• CD4 is a T-cell surface glycoprotein also known as L3T4 or Ly-4, encoded by the Cd4 gene in mouse.
• It functions as a coreceptor for MHC class II molecules, interacting with the T-cell receptor (TCR) and MHC II to enhance T-cell activation.
• Upon ligand binding, CD4 recruits the Src kinase LCK to the TCR-CD3 complex, initiating intracellular signaling pathways leading to T-helper cell proliferation, adhesion, and cytokine production.
• In macrophages and NK cells, CD4 mediates differentiation, activation, and migration via TCR-independent mechanisms.
• CD4 is predominantly expressed on the surface of T-helper cells, but is also found on macrophages, dendritic cells, and NK cells.
• The protein is post-translationally modified with glycosylation, disulfide bonds, and palmitoylation, and has a predicted molecular mass of ~51 kDa (UniProt: 51297 Da).
• It is a single-pass type I membrane protein localized to the cell membrane.

Experimental Guidance and Technical Tips

• The immunogen covers the extracellular domain (amino acids 27-394) of mouse CD4, which includes the MHC class II binding region; consider this design when evaluating epitope accessibility.
• For flow cytometry, consider titrating the antibody with appropriate isotype controls to optimize signal-to-noise ratio in your specific sample type.
• Validate staining conditions on live versus fixed cells as needed for your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting. For CD4-related immune cell profiling and T-helper cell studies, CamelBio offers validated antibody pairs, optimized monoclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to support your development from concept to clinic.