Anti-Mouse CD34 Monoclonal Antibody for Flow Cytometry - Q64314

Core Product Specifications and Parameters

Biological Background: CD34 Function and Localization

• CD34 is a single-pass type I membrane protein and member of the sialomucin family, encoded by the Cd34 gene in mouse. Alternative splicing generates multiple isoforms.
• Acts as a possible adhesion molecule in early hematopoiesis, mediating stem cell attachment to the bone marrow extracellular matrix or stromal cells. It may scaffold lineage-specific glycans to present carbohydrate ligands to selectins (By similarity).
• Subcellular localization is predominantly at the cell membrane, consistent with its role as a transmembrane receptor.
• Tissue expression: Detected in kidney, particularly the thin limb of Henle's loop (at protein level). Highly expressed in hematopoietic progenitor cell lines, brain, and testis; moderate in thymus, spleen, and bone marrow; absent in adult liver. Related references: PMID:31605441 PMID:1709048
• Classified as a glycoprotein and phosphoprotein, with a signal peptide and a single transmembrane helix.
• Known to participate in cell adhesion and signal transduction, influencing stem cell fate decisions.
• The protein is heavily glycosylated, contributing to its observed molecular weight of ~41 kDa.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (aa 35–287), maximizing accessibility for live-cell flow cytometry. No permeabilization is required.
• Optimize staining by titrating the antibody over a concentration range (e.g., 0.1–5 μg/mL) in your specific experimental system, and include appropriate isotype controls.
• Because the antibody is directly conjugated to , no secondary antibody is needed, reducing background and preparation steps.
• For quantitative FC, consider using calibration beads to convert fluorescence intensity to molecules of equivalent soluble fluorochrome (MESF).
• Antigen density may vary across cell types; validate the antibody on known positive (e.g., hematopoietic progenitor cells) and negative populations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers, laboratories, and research institutes with a complete portfolio of IVD raw materials, bridging concepts to the clinic. This anti-mouse CD34 monoclonal antibody supports core hematopoiesis and stem cell research, and we can additionally provide validated antibody pairs, optimized monoclonal antibodies, bulk ancillary reagents, and sourcing of rare targets. Let CamelBio be your reliable partner for all IVD raw-material needs.