Anti-Mouse CD317/BST2 Rabbit Monoclonal Antibody for WB and ELISA - Q8R2Q8

Core Product Specifications and Parameters

Biological Background: CD317/BST2 Function and Localization

• Also known as bone marrow stromal antigen 2, HM1.24 antigen, GREG, Bst-2, CD317, and DAMP-1, encoded by the Bst2 gene in mouse.
• Acts as an interferon-induced antiviral restriction factor that directly tethers nascent enveloped virions to the cell membrane, preventing their release and spread as cell-free particles.
• Targets a broad range of enveloped viruses, including HIV-1, MMTV, MLV, Ebola virus, Lassa virus, and VSV, by physically linking virions to the cell surface.
• Inhibits cell surface proteolytic activity of MMP14, leading to decreased MMP15 activation and subsequent reduction in cell growth and migration.
• Stimulates signaling by LILRA4/ILT7 on plasmacytoid dendritic cells, providing negative feedback to type I interferon production during viral infection.
• Localizes to the Golgi apparatus, trans-Golgi network, plasma membrane, late endosomes, membrane rafts, and the apical surface of polarized epithelial cells.
• In naive mice, expression is specific to type I interferon-producing cells at the protein level, highlighting its role in innate immunity.

Experimental Guidance and Technical Tips

• The immunogen spans the extracellular domain (amino acids 55–152), which is highly exposed and conserved; this should yield antibodies that recognize the native protein in most applications.
• Validated for Western blot and ELISA; consider optimizing antibody dilution and incubation conditions for your specific sample type and detection system.
• As a transmembrane/GPI-anchored protein, reduce samples with DTT or beta-mercaptoethanol for Western blot to ensure proper denaturation and migration.
• For immunohistochemistry or immunofluorescence, perform careful validation due to the protein’s membrane localization and potential epitope masking in fixed tissues.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, supporting diagnostic manufacturers and research laboratories from concept to clinic. This anti-CD317/BST2 antibody is part of our growing portfolio of validated reagents for antiviral and innate immunity workflows. We offer one-stop access to optimized antibody pairs, monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing services to accelerate your assay development.