Anti-Mouse CD317/BST2 Monoclonal Antibody for Flow Cytometry - Q8R2Q8

Core Product Specifications and Parameters

Biological Background: CD317/BST2 Function and Localization

• Bone marrow stromal antigen 2 (BST2), also known as CD317 or HM1.24 antigen, is a lipid raft-associated membrane protein encoded by the Bst2 gene in mice.
• Functions as an interferon-induced antiviral restriction factor that blocks the release of diverse enveloped viruses by directly tethering nascent virions to the cell membrane (e.g., HIV-1, MLV, EBOV, VSV).
• Exhibits a broad antiviral spectrum, restricting retroviruses, filoviruses, arenaviruses, and rhabdoviruses.
• Localizes to the trans-Golgi network, cell membrane, late endosomes, membrane rafts, and apical cell membrane; also found in the cytoplasm.
• Specifically expressed on type I interferon-producing cells (plasmacytoid dendritic cells) in naive mice at the protein level.
• Contains a signal-anchor transmembrane domain, coiled-coil region, and a GPI-anchor; post-translational modifications include disulfide bonds, N-glycosylation, and lipidation.
• Plays a role in organizing the subapical actin cytoskeleton in polarized epithelial cells and can inhibit MMP14-mediated cell growth and migration.
• Provides negative feedback on IFN production by stimulating LILRA4/ILT7 signaling in plasmacytoid dendritic cells.

Experimental Guidance and Technical Tips

• The immunogen used (amino acids 55-152 of mouse BST2) corresponds to the extracellular domain, which may be suitable for detecting surface expression by flow cytometry.
• Since this antibody is directly conjugated to , no secondary antibody staining is needed; consider using appropriate isotype control conjugates to ensure specificity.
• Validate staining in known BST2-expressing mouse cell types, such as plasmacytoid dendritic cells or type I interferon-producing cells.
• For flow cytometry, titrate the antibody to determine optimal concentration for your specific sample and avoid non-specific binding.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. This anti-mouse CD317/BST2 antibody supports research in antiviral immunity and viral infection models, and we can assist with additional related reagents or custom raw material sourcing to accelerate your diagnostic development.