Anti-Mouse CD3 Monoclonal Antibody for Flow Cytometry - P04235 / P22646

Core Product Specifications and Parameters

Biological Background: CD3 Function and Localization

• The target protein, CD3δ (T-cell surface glycoprotein CD3 delta chain), is a core component of the TCR-CD3 complex present on the surface of T lymphocytes, where it plays an essential role in adaptive immune responses.
• Together with CD3ε, CD3γ, and CD247/CD3ζ, CD3δ transmits TCR-mediated signals across the cell membrane upon antigen recognition by the T-cell receptor (TCR), triggering downstream signaling pathways.
• All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domains. Upon TCR engagement, these ITAMs are phosphorylated by Src family kinases LCK and FYN, leading to activation of signaling cascades.
• CD3δ plays a critical role in thymocyte differentiation by ensuring correct intracellular assembly and surface expression of the TCR-CD3 complex; without a functional complex, thymocytes fail to differentiate properly. Related references: PMID:10935641
• CD3δ interacts with the coreceptors CD4 and CD8, establishing a functional link between the TCR and these molecules, which is required for activation and positive selection of CD4+ or CD8+ T cells.
• The CD3δ protein is a membrane-spanning glycoprotein with a signal peptide, a single transmembrane helix, and a cytoplasmic ITAM-containing domain. It is subject to phosphorylation and disulfide bond formation.
• CD3ε, the second target recognized by this antibody, shares structural and functional similarities with CD3δ and is also part of the TCR-CD3 complex, contributing to signal transduction and T-cell development.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide derived from mouse CD3, suggesting that the antibody likely recognizes a linear epitope conserved between the δ and ε chains, consistent with its dual reactivity.
• For flow cytometry (FC), consider titrating the antibody to determine the optimal concentration for your specific mouse lymphocyte population. Validate staining in both resting and activated T-cell samples.
• As this is a rat monoclonal antibody, ensure appropriate anti-rat secondary detection reagents or direct conjugation strategies if multiplexing with other antibodies.
• Due to the predicted molecular weights (19 kDa for CD3δ, 21 kDa for CD3ε), the antibody may also be suitable for Western blotting under denaturing conditions, though the manufacturer’s data supports FC. Consider in-house validation if exploring additional applications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with comprehensive access to IVD raw materials, technical services, and consulting—supporting projects from concept to clinic. For immunology-focused research and assay development, we can assist in sourcing this anti-CD3 antibody along with validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and even rare-target raw materials tailored to your specific needs.