Anti-Mouse CD3 Monoclonal Antibody for FC - P04235 / P22646

Core Product Specifications and Parameters

Biological Background: CD3 Delta Chain Function and Localization

• CD3 delta is a crucial subunit of the TCR-CD3 complex on T-lymphocytes, indispensable for adaptive immune responses. This antibody also recognizes CD3 epsilon (P22646), another key CD3 chain.
• Upon antigen presentation, the TCR-CD3 complex transduces activation signals across the membrane via the CD3 chains (delta, epsilon, gamma, and zeta), each containing immunoreceptor tyrosine-based activation motifs (ITAMs).
• ITAMs in the cytoplasmic domain of CD3 delta are phosphorylated by Src family kinases LCK and FYN upon TCR engagement, initiating downstream signaling cascades.
• CD3 delta is essential for proper thymocyte differentiation by ensuring correct assembly and surface expression of the TCR-CD3 complex; without it, thymocyte development is impaired. Related references: PMID:10935641
• It functionally interacts with CD4 and CD8 coreceptors, linking the TCR to these molecules and facilitating T-cell activation and positive selection.
• Subcellular localization is primarily the plasma membrane; it is a single-pass transmembrane protein with extracellular, transmembrane, and cytoplasmic domains.
• CD3 delta is a glycoprotein and phosphoprotein, playing a central role in receptor signaling and immune cell development.

Experimental Guidance and Technical Tips

• This antibody is validated for flow cytometry (FC). Consider titrating the antibody to determine the optimal concentration for your specific sample and experimental conditions.
• Include appropriate isotype controls and perform Fc receptor blocking to reduce nonspecific binding in mouse cell staining.
• As a rat-derived monoclonal antibody, use anti-rat secondary reagents if performing indirect detection; for direct detection, the conjugate is pre-validated.
• The immunogen is a synthetic peptide; while the antibody performs well in FC under native conditions, its suitability for other applications (e.g., WB, IHC) may require additional validation in your model system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research laboratories streamlined access to a comprehensive portfolio of IVD raw materials, including validated monoclonal and polyclonal antibodies, antibody pairs, and supporting reagents. For T-cell biology and immunophenotyping workflows, this anti-mouse CD3 antibody is one of many high-quality options available, backed by our ability to source rare targets and provide bulk custom productions. Partner with CamelBio to bridge your project from concept to clinic with reliable, one-stop procurement solutions.