Anti-Mouse CD274 (PD-L1) Rabbit Monoclonal Antibody for WB, IF/ICC, IHC-P, FC, ELISA - Q9EP73

Core Product Specifications and Parameters

Biological Background: CD274/PD-L1 Function and Localization

• CD274, also known as PD-L1 or B7-H1, is a type I transmembrane protein belonging to the immunoglobulin superfamily.
• Acts as a ligand for the inhibitory receptor PD-1 (PDCD1) to play a critical role in maintaining immune tolerance by modulating T-cell activation thresholds and limiting effector responses. Related references: PMID:11238124
• Via a yet unknown activating receptor, may costimulate T-cell subsets that predominantly produce interleukin-10 (IL-10). Related references: PMID:11015443 PMID:12719480
• The PD-1/PD-L1 pathway is exploited by tumors to attenuate anti-tumor immunity; interaction with PD-1 inhibits cytotoxic T lymphocyte (CTL) effector function. Related references: PMID:12218188
• Blockade of PD-1/PD-L1 signaling reverses T-cell exhaustion and normalizes anti-tumor responses, providing the basis for immune checkpoint cancer immunotherapy. Related references: PMID:12218188
• Localizes primarily to the cell membrane, and can recycle via early and recycling endosomes.
• Highly expressed in heart, thymus, skeletal muscle, and lung; weakly in kidney, spleen, thyroid, liver. Expressed on activated dendritic cells, B-cells, macrophages, and numerous tumor cell lines of lymphoid origin.

Experimental Guidance and Technical Tips

• For Western blot, the predicted molecular weight is ~33 kDa, but glycosylation may result in a band around 40–50 kDa. Validate in the relevant sample system.
• In IHC-P, consider antigen retrieval and signal amplification due to variable expression levels; validate with appropriate positive and negative tissues.
• For flow cytometry, use on viable cells to detect surface PD-L1; include appropriate isotype controls and confirm detection in your cellular system.
• This antibody recognizes mouse CD274; cross-reactivity with other species has not been tested. Confirm if required for your experimental model.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutes with reliable access to IVD raw materials, from validated antibody pairs and optimized monoclonal/polyclonal antibodies to bulk ancillary reagents. For immune checkpoint targets like CD274/PD-L1, our supply capabilities support your assay development across applications such as IHC, flow cytometry, and ELISA. Whether you need single antibodies or complete reagent sets, CamelBio is your partner from concept to clinic.