Anti-Mouse CD272/BTLA Monoclonal Antibody () for Flow Cytometry - Q7TSA3

Core Product Specifications and Parameters

Biological Background: CD272/BTLA Function and Localization

• CD272 (BTLA) is a B- and T-lymphocyte attenuator, an inhibitory receptor belonging to the immunoglobulin superfamily. It contains an extracellular immunoglobulin domain, a transmembrane helix, and a cytoplasmic tail with immunoreceptor tyrosine-based inhibitory motifs (ITIMs).
• BTLA negatively regulates antigen receptor signaling in lymphocytes by recruiting protein tyrosine phosphatases SHP-1 and SHP-2. Related references: PMID:12796776 PMID:14652006
• It interacts with TNFRSF14 (HVEM) in cis (on the same cell) or in trans (between cells). Cis interactions maintain a resting state in naive T cells, while trans interactions provide survival signals to effector T cells during adaptive immune responses. Related references: PMID:19915044
• Expressed in splenic T- and B-cells, lymph node tissues, and also in macrophages, NK cells, and dendritic cells. A polymorphic tissue distribution is observed between different mouse strains.
• The protein is located at the cell membrane as a single-pass type I membrane protein, with an N-terminal signal peptide cleaved during processing.
• Post-translational modifications include glycosylation and phosphorylation, and its structure contains disulfide bonds important for immunoglobulin domain stability.
• Alternative splicing generates multiple isoforms, though the functional significance in mouse is not fully characterized.
• Broadly involved in adaptive immunity, with three-dimensional structural data available.

Experimental Guidance and Technical Tips

• The antibody is directly conjugated to , a red fluorescent dye, suited for flow cytometric detection. Consider titrating the antibody to determine the optimal staining concentration for your experimental system.
• Generated against the extracellular domain (amino acids 30-176), it likely recognizes the native cell surface receptor. Include appropriate isotype controls to ensure specificity.
• For staining immune cells, combine with lineage markers to gate on specific populations (e.g., T cells, B cells, NK cells).
• Standard flow cytometry staining protocols (e.g., 30 min at 4°C) are applicable, but validate fixation steps as they may affect epitope recognition.
• As a rabbit monoclonal antibody, it can be detected with an anti-rabbit IgG secondary reagent if needed, though direct conjugation eliminates this step.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with a comprehensive range of IVD raw materials and services. This Rabbit anti-Mouse CD272/BTLA monoclonal antibody supports immunology and oncology research, enabling precise detection of BTLA by flow cytometry. Beyond high-performance antibodies, CamelBio offers validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to streamline your development pipeline from concept to clinic.