Anti-Mouse CD252 (OX40 Ligand) Monoclonal Antibody for IF/ICC, FC - P43488

Core Product Specifications and Parameters

Biological Background: OX40 Ligand Function and Localization

• The protein encoded by the Tnfsf4 gene, also known as OX40 ligand (OX40L) or CD252, is a member of the tumor necrosis factor (TNF) ligand superfamily (TNFSF4). It is also designated as CD134L or gp34.
• OX40 ligand functions as a cytokine that binds to its receptor TNFRSF4 (OX40) on T-cells, providing co-stimulatory signals that promote T-cell proliferation and cytokine production.
• The protein is a type II transmembrane protein, anchored in the membrane with an N-terminal cytoplasmic domain and a C-terminal extracellular TNF homology domain.
• Post-translational modifications include N-linked glycosylation and a conserved disulfide bond typical of the TNF family, which is essential for its structural integrity and receptor binding.
• Subcellular localization is primarily on the plasma membrane of activated antigen-presenting cells, such as dendritic cells and B cells, and it can also be expressed on endothelial cells.
• This mouse protein shares significant homology with human OX40 ligand, and it plays a critical role in T-cell-dependent immune responses and memory T-cell generation.

Experimental Guidance and Technical Tips

• This rabbit monoclonal antibody was generated using a recombinant immunogen covering amino acids 51-198 of mouse CD252, which corresponds to the extracellular domain. This region is predicted to present native epitopes exposed on the cell surface.
• For IF/ICC assays, consider that the antigen is membrane-bound; gentle fixation and permeabilization may be required to preserve epitope accessibility. Validation in a positive cell line expressing mouse OX40 ligand is recommended.
• In flow cytometry (FC), the antibody is expected to detect surface-expressed OX40 ligand on activated mouse cells. A matched isotype control and appropriate Fc receptor blocking should be included to ensure specificity.
• Avoid repeated freeze-thaw cycles, and store as recommended (shipped on ice bags) to maintain antibody stability and activity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines procurement for diagnostic developers and researchers by offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. This anti-mouse CD252 (OX40 Ligand) monoclonal antibody is ideal for T-cell co-stimulation studies and immune profiling. For specialized needs, our team can assist in sourcing rare-target raw materials to accelerate your project from concept to clinic.