Anti-Mouse CD25 Monoclonal Antibody, Conjugate, for Flow Cytometry - P01590

Core Product Specifications and Parameters

Biological Background: CD25 Function and Localization

• Interleukin-2 receptor subunit alpha (CD25), also known as p55 or Ly-43, is the high-affinity IL-2 receptor alpha chain.
• CD25 forms a heterotrimeric receptor complex with IL-2 receptor beta (CD122) and common gamma chain (CD132) to bind interleukin-2 with high affinity.
• Essential for regulatory T cell (Treg) function; involved in maintaining immune tolerance by controlling Treg activity and suppressing autoreactive T-cell expansion.
• Localized to the plasma membrane as a type I transmembrane protein, with an extracellular region containing Sushi domains and a signal peptide.
• Post-translational modifications include N-linked glycosylation and disulfide bond formation.
• Belongs to the type I cytokine receptor family; characterized by a transmembrane helix and intracellular signaling motifs.

Experimental Guidance and Technical Tips

• The antibody is generated against a recombinant fusion protein encompassing amino acids 22–236 of mouse CD25, which corresponds to the full extracellular domain. This target region includes the IL-2 binding site and Sushi domains.
• For flow cytometry, it is recommended to titrate the antibody to determine the optimal staining concentration for each cell type and experimental condition.
• As the antibody is conjugated to , no secondary antibody is required; ensure the flow cytometer has appropriate excitation and emission filters (e.g., 561 nm laser, 610/20 nm bandpass).
• Use appropriate isotype controls and include Fc receptor blocking steps to minimize nonspecific binding, especially when analyzing primary mouse immune cells.
• The antibody is validated for mouse reactivity; cross-reactivity with other species has not been tested.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides one-stop access to validated IVD raw materials and custom sourcing services, including this anti-mouse CD25 monoclonal antibody for immune tolerance and Treg research. We support diagnostic manufacturers and laboratories with bulk supply of conjugated antibodies, antigen reagents, and technical consultation to accelerate immunoassay development and clinical translation.