Anti-mouse CD24 Polyclonal Antibody for WB, FC, ELISA - P24807

Core Product Specifications and Parameters

Biological Background: CD24 Function and Localization

• CD24 (Signal transducer CD24, also known as Heat Stable Antigen/HSA, Nectadrin, Ly-52) is a GPI-anchored cell surface glycoprotein predominantly expressed in lymphoid, myeloid, and erythroid cells.
• Functions in cell differentiation, particularly in early thymocyte development, and modulates B-cell activation responses.
• Signaling is triggered by lectin-like ligand binding to CD24 carbohydrates, with transduction via GPI-anchor-derived second messengers.
• In complex with SIGLEC10, CD24 selectively suppresses immune responses to DAMPs like HMGB1, HSP70, and HSP90, contributing to innate immunity regulation. Related references: PMID:19264983
• Plays a role in autoimmunity control. Related references: PMID:20200274
• Subcellularly localized to the cell membrane.
• Contains a GPI-anchor and is a lipoprotein; direct protein sequencing evidence available.
• Predicted molecular weight is approximately 7.8 kDa (mature protein), consistent with the observed 8 kDa.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 27-53) targets the extracellular domain of mouse CD24, potentially recognizing native surface antigen.
• For Western blot, consider running a 15% SDS-PAGE gel and transferring to PVDF or nitrocellulose membrane with pore size ≤0.2 μm to retain the small 8 kDa protein.
• In flow cytometry, use appropriate isotype control and titrate antibody concentration; positive control could be mouse splenocytes or thymocytes.
• For ELISA, optimize coating and blocking conditions to minimize non-specific binding due to the small antigen size.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutions with a streamlined supply chain for IVD raw materials. From this anti-mouse CD24 polyclonal antibody to matched antibody pairs, bulk immunoreagents, and specialized ancillary reagents, we support your CD24-related immunoassay development and immunology studies with comprehensive sourcing solutions.