Anti-Mouse CD23 Monoclonal Antibody for FC - P20693

Core Product Specifications and Parameters

Biological Background: Mouse CD23 Function and Localization

• Mouse CD23, also known as Fc-epsilon-RII or lymphocyte IgE receptor, is a low-affinity receptor for immunoglobulin E (IgE) and complement receptor 2 (CR2/CD21).
• It plays a pivotal role in regulating IgE production and B-cell differentiation, and facilitates IgE-dependent antigen uptake and presentation to T cells.
• On macrophages, IgE-bound CD23 cross-linking activates the L-arginine–nitric oxide pathway, leading to intracellular killing of parasites.
• The receptor exists as both membrane-bound and secreted isoforms via alternative splicing, localizing primarily to the cell membrane but also found in a soluble form.
• CD23 is a type II transmembrane glycoprotein modified by palmitoylation, disulfide bonds, and glycosylation; it features a C-type lectin domain, calcium-binding sites, and a proteoglycan form.

Experimental Guidance and Technical Tips

• The immunogen spans the extracellular domain (aa 50–331), so the antibody is expected to recognize both membrane-bound and soluble CD23.
• For flow cytometry, titrate the antibody to determine the optimal concentration for your specific cell type and staining conditions.
• Due to CD23 shedding, consider using fresh samples or including a shedding inhibitor for detection of surface expression.
• Verify expression of CD23 in your cell system, as it is primarily expressed on B cells and activated macrophages.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic developers and researchers with streamlined access to IVD raw materials, including validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents for immunology and allergy studies. For CD23-focused projects, we leverage our supply network to source rare-target raw materials and support your workflow from early R&D to clinical application. Partner with CamelBio to simplify procurement and accelerate your diagnostic development.