Anti-Mouse CD223/LAG-3 Monoclonal Antibody for FC - Q61790

Core Product Specifications and Parameters

Biological Background: Lymphocyte activation gene 3 protein Function and Localization

• LAG-3 functions as an inhibitory receptor on antigen-activated T-cells, delivering suppressive signals upon engagement with its ligands MHC class II on antigen-presenting cells and FGL1 secreted by hepatocytes and tumor cells. Related references: PMID:12209638, PMID:12421911, PMID:12672063, PMID:30349037
• Ligand binding induces ubiquitination of the KIEELE motif, releasing the RRFSALE motif and promoting the formation of condensates with the TCR component CD3E, thereby disrupting CD3E-LCK association and inhibiting TCR signaling. Related references: PMID:40101708, PMID:40592325
• LAG-3 may synergize with PD-1/PDCD1 to inhibit antigen-specific T-cell activation. Related references: PMID:21300912
• It negatively regulates proliferation, activation, effector functions, and homeostasis of both CD8+ and CD4+ T-cells. Related references: PMID:12209638, PMID:12421911, PMID:12672063
• Constitutively expressed on a subset of regulatory T-cells (Tregs), LAG-3 contributes to their suppressive function and also acts as a negative regulator of plasmacytoid dendritic cell (pDC) activation. Related references: PMID:15485628, PMID:19201841
• The LAG-3 inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity, highlighting its role as an immune checkpoint target.
• Primarily expressed in activated CD4+ and CD8+ T-cells, with additional expression in a subset of Tregs and pDCs. Related references: PMID:12209638, PMID:15485628, PMID:19201841
• LAG-3 localizes to the cell membrane and can also be secreted.

Experimental Guidance and Technical Tips

• When using this -conjugated antibody for flow cytometry, consider titrating on activated mouse splenocytes or T-cell lines to determine optimal staining concentrations.
• The immunogen covers the extracellular domain (aa 24–442), which may ensure recognition of the native receptor; verify performance on live, unfixed cells.
• As LAG-3 is an activation marker, in vitro stimulation of T-cells (e.g., with anti-CD3/CD28) may be required to upregulate expression before analysis.
• For intracellular staining, evaluate compatibility with fixation/permeabilization protocols, as the fluorophore may be sensitive to certain buffers.
• Include appropriate isotype controls and, if multiplexing, check for potential compensation issues with the dye.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials, including validated antibody pairs and optimized monoclonal antibodies such as this anti-LAG-3 clone, tailored for immuno-oncology assay development. Our one-stop procurement services encompass bulk ancillary reagents, rare target sourcing, and technical consultation, streamlining your workflow from concept to clinic. Whether you require custom antibody generation or ready-to-use critical raw materials, CamelBio bridges the gap between discovery and diagnostic commercialization.