Anti-Mouse CD22 Rabbit Monoclonal Antibody for Flow Cytometry - P35329

Core Product Specifications and Parameters

Biological Background: CD22 Function and Localization

• CD22 (Siglec-2, BL-CAM) is the most highly expressed siglec on B-cells, playing a critical role in B-cell differentiation, antigen presentation, and bone marrow trafficking. Related references: PMID:7533044 PMID:9016707
• It functions as an inhibitory coreceptor of the B-cell receptor (BCR), dampening BCR-induced calcium mobilization and cellular activation. Related references: PMID:9016707
• The immunoreceptor tyrosine-based inhibitory motif (ITIM) of CD22 is phosphorylated by Src family kinase LYN, leading to recruitment of protein tyrosine phosphatase SHP-1 (PTPN6) and negative regulation of BCR signaling.
• Strong negative signaling through CD22 can induce B-cell apoptosis.
• CD22 binds α2,6-linked sialic acid residues on cis surface molecules (CD22, CD45, IgM) and may mediate trans cell adhesion through sialic acid binding.
• The protein is expressed specifically in B-lymphocytes and localizes to the cell membrane.
• Alternative splicing generates multiple isoforms; the canonical isoform of mouse CD22 has a predicted molecular weight of approximately 97.2 kDa (UniProt P35329).

Experimental Guidance and Technical Tips

• The immunogen (aa 22–151) targets the extracellular N-terminal region, including the Ig-like V-set domain, suggesting the antibody can recognize native surface CD22 on mouse B-cells in flow cytometry.
• For best results, titrate the antibody and include appropriate isotype controls and Fc receptor blocking steps when staining primary B-cells or B-cell lines.
• CD22 expression is restricted to B-lymphocytes; validate specificity using B-cell positive and T-cell/other lineage negative controls.
• The conjugate is compatible with common violet/UV laser lines; avoid buffers containing high autofluorescence or preservatives that may interfere with the dye.
• Ship and store under ice bag conditions to maintain conjugate stability; protect from light.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research institutions a streamlined channel to secure high-quality IVD raw materials like this anti-CD22 antibody. With capabilities ranging from validated monoclonal antibodies and recombinant proteins to customized bulk ancillary reagents, we support B-cell targeted assay development and immunology research from concept to clinic. Whether you need optimized antibody pairs or rare target sourcing, CamelBio is your strategic partner for scalable, research-grade immunoreagents.