Anti-Mouse CD22 Monoclonal Antibody () for IF-P, FC - P35329

Core Product Specifications and Parameters

Biological Background: B-cell receptor CD22 Function and Localization

• CD22, also known as Siglec-2 and B-lymphocyte cell adhesion molecule (BL-CAM), is a sialic acid-binding immunoglobulin-like lectin predominantly expressed on B-cells.
• It functions as an inhibitory coreceptor on the surface of B-cells, negatively modulating B-cell receptor (BCR) signaling by inhibiting calcium mobilization and cellular activation, and under strong inhibitory signals can induce apoptosis. Related references: PMID:7533044 PMID:9016707
• Mechanistically, the immunoreceptor tyrosine-based inhibitory motif (ITIM) is phosphorylated by the Src kinase LYN, leading to recruitment of the protein tyrosine phosphatase PTPN6/SHP-1, which dampens downstream signaling. Related references: PMID:9016707
• CD22 binds alpha-2,6-linked sialic acid residues on surface molecules such as CD22 itself, CD45, and IgM in cis, and can also mediate cell adhesion via trans interactions with ligands on other cells.
• The protein is specifically localized to the cell membrane and is expressed in B-lymphocyte lineages.
• Multiple isoforms are produced by alternative splicing, contributing to distinct molecular weights (e.g., 89kDa, 93kDa, 97kDa).
• CD22 is a glycoprotein and phosphoprotein, with N-glycosylation and tyrosine phosphorylation playing critical roles in its function.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (aa 22-151), making this antibody suitable for detecting surface CD22 in immunofluorescence and flow cytometry. Ensure minimal permeabilization if targeting surface epitopes.
• For flow cytometry, titrate the antibody on mouse B-cell lines or primary splenocytes to determine optimal concentration, using appropriate isotype controls.
• is a bright and photostable far-red fluorophore; avoid prolonged light exposure and use suitable filter sets (e.g., 633 nm excitation, 660 nm emission).
• Consider fixing cells with 4% PFA for IF-P; if co-staining, validate fixation compatibility with other antibodies.
• Cross-reactivity is reported for mouse; verify reactivity in the relevant sample system if used on other species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research institutes integrated access to high-quality IVD raw materials, including validated monoclonal and polyclonal antibodies, antigen pairs, and bulk ancillary reagents. This anti-mouse CD22 antibody supports B-cell immunophenotyping and lymphoma research applications. For projects requiring rare-target raw materials or customized antibody sourcing, CamelBio provides expert consulting and supply chain support from concept to clinic.