Anti-Mouse CD22 Monoclonal Antibody () for Flow Cytometry - P35329

Core Product Specifications and Parameters

Biological Background: CD22 Function and Localization

• CD22 (Siglec-2, BL-CAM, Lyb-8) is the most highly expressed sialic acid-binding immunoglobulin-like lectin on B-cells, crucial for B-cell differentiation, antigen presentation, and bone marrow trafficking.
  Related references:
  PMID:7533044, PMID:9016707
• It binds α2,6-linked sialic acid residues on surface molecules (e.g., CD45, IgM, CD22) in a cis configuration, and can also mediate cell adhesion via trans interactions with ligands on other cells.
• As an inhibitory coreceptor, CD22's ITIM domain is phosphorylated by LYN kinase upon BCR engagement, recruiting SHP-1 (PTPN6) to suppress BCR-induced calcium mobilization and cellular activation.
  Related references:
  PMID:9016707
• Strong CD22-mediated negative signaling may lead to B-cell apoptosis.
• CD22 is exclusively localized to the plasma membrane of B-lymphocytes.
• Multiple isoforms exist due to alternative splicing, with predicted molecular weights of 89, 93, and 97 kDa; the protein is glycosylated and contains disulfide bonds.
• Functional keywords: Cell adhesion, Lectin, Immunoglobulin domain, Phosphoprotein, Alternative splicing, Glycoprotein, Signal, Transmembrane.

Experimental Guidance and Technical Tips

• The conjugate allows direct flow cytometric detection without a secondary antibody; titrate the antibody to determine the optimal concentration for your specific experimental system.
• Since the immunogen corresponds to the extracellular ligand-binding domain (aa 22-151), this antibody is suitable for staining live, unfixed B-cells; consider including Fc receptor blocking steps to reduce non-specific binding.
• Validate expression in the relevant B-cell population (e.g., splenocytes, lymph node cells, or B-cell lines) and use appropriate isotype controls and knockout/knockdown controls if available.
• Due to alternative splicing, verify that the antibody recognizes the isoform(s) expressed in your sample; molecular weight shifts may be observed in western blotting if adapted.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive procurement partner for IVD manufacturers and research laboratories, offering a wide range of validated antibodies, bulk reagents, and ancillary raw materials. For B-cell biology and immunological studies, we supply optimized monoclonal antibodies, including conjugated formats like this anti-CD22 , along with rare-target sourcing solutions to accelerate your diagnostic development from concept to clinical application.