Anti-Mouse CD215/IL-15Rα Monoclonal Antibody () for IF/ICC, FC - Q60819

Core Product Specifications and Parameters

Biological Background: CD215/IL-15Rα Function and Localization

• High-affinity receptor for interleukin-15 (IL-15). Mediates cis and trans signaling; in trans, IL15RA presents IL-15 to neighboring IL2RG-expressing cells. Related references: PMID:17947230
• In neutrophils, binds and activates kinase SYK in response to IL-15 stimulation.
• Required for IL-15-induced phagocytosis in neutrophils in a SYK-dependent manner.
• Subcellular localization includes the cell membrane, nuclear membrane, and cell surface; also secreted into the extracellular space.
• Widely expressed across mouse tissues.
• Post-translational modifications: glycoprotein, phosphoprotein; contains a signal peptide, sushi domain, and transmembrane helix; undergoes alternative splicing.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 33–205) corresponds to the extracellular domain of mouse CD215/IL-15Rα, likely recognizing the native receptor on the cell surface.
• For immunofluorescence (IF/ICC) or flow cytometry (FC), consider using appropriate cell fixation and permeabilization protocols, especially to detect nuclear membrane-localized receptor.
• As an -conjugated antibody, it enables direct detection without secondary antibodies; validate in the relevant mouse sample system.
• Since IL15RA can be secreted, consider including blocking steps to distinguish between membrane-bound and soluble forms.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents—covering every stage from concept to clinic. For studying interleukin-15 signaling and immune cell function, we offer reliable sourcing of CD215/IL-15Rα antibodies and related raw materials, along with technical services and consulting to support rare target procurement.