Anti-Mouse CD215/IL-15Rα Monoclonal Antibody for IF/ICC, FC - Q60819

Core Product Specifications and Parameters

Biological Background: CD215/IL-15Rα Function and Localization

• IL15RA encodes the interleukin-15 receptor subunit alpha, also known as CD215.
• Functions as the high-affinity receptor for IL-15, mediating both cis and trans signaling. Related references: PMID:17947230
• In neutrophils, interacts with and activates kinase SYK upon IL-15 stimulation, and is required for IL-15-induced phagocytosis.
• Contains a sushi domain (short consensus repeat) in the extracellular region; undergoes alternative splicing to produce multiple isoforms.
• Localizes to the cell membrane and nucleus membrane, and can be secreted into the extracellular space.
• Widely expressed in various tissues.
• Post-translational modifications include glycosylation, phosphorylation, and disulfide bond formation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (aa 33-205), suggesting the antibody recognizes the ligand-binding region; consider this when designing blocking or competition experiments.
• For IF/ICC, optimize fixation and permeabilization protocols for your mouse cell type; the conjugate permits direct fluorescence detection without a secondary antibody.
• In flow cytometry, titrate the antibody to determine the optimal staining concentration for your cell population; use appropriate isotype controls.
• Validate reactivity in the relevant mouse sample systems, including cell lines or primary cells known to express IL-15Rα.
• Note that IL-15Rα can be shed or secreted; staining protocols may need to account for both membrane-bound and soluble forms.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with comprehensive access to IVD raw materials, from validated antibody pairs and optimized monoclonal antibodies to bulk ancillary reagents. For targets like CD215/IL-15Rα involved in cytokine signaling and immune regulation, our sourcing capabilities extend to rare and custom reagents. Our platform streamlines procurement for every stage, from concept to clinic, ensuring you receive reliable materials for your assay development.