Anti-Mouse CD204 Monoclonal Antibody for Flow Cytometry - P30204

Core Product Specifications and Parameters

Biological Background: CD204 (MSR1) Function and Localization

• Mediates binding, endocytosis, and phagocytosis of diverse ligands including acetylated LDL (AcLDL) and oxidized LDL (OxLDL), promoting cholesterol uptake and foam cell formation in atherosclerosis. Related references: PMID:9348300 PMID:10722588
• Recognizes pathogen-associated molecular patterns and apoptotic cell debris, supporting macrophage clearance activity in innate immune defense. Related references: PMID:9348300 PMID:10722588
• Upon CpG DNA stimulation, mediates LYN activation, which phosphorylates SYK; phosphorylated SYK is recruited to endosomal TLR9 to promote downstream TLR9 signaling. Related references: PMID:9348300 PMID:10722588
• Alternative splicing generates macrophage scavenger receptor types I and II (SR-AI/SR-AII), both featuring collagen-like, coiled-coil, and transmembrane domains.
• Expressed in monocyte-derived macrophages, consistent with its scavenging function at inflammatory sites.
• Key post-translational modifications include glycosylation and phosphorylation; contains a signal-anchor, transmembrane helix, and disulfide bonds.

Experimental Guidance and Technical Tips

• The immunogen spans the collagenous domain (aa 350-458); consider verifying antibody reactivity with full-length CD204 or native receptor on macrophages.
• For flow cytometry, titrate the optimal staining concentration using mouse macrophage cell lines (e.g., RAW 264.7) or primary mouse macrophages as positive controls.
• direct conjugation enables multi-color panel design; ensure proper compensation with other fluorophores in your flow cytometry assay.
• Validate detection of endogenous CD204 in your specific sample system (e.g., thioglycollate-elicited peritoneal macrophages) to confirm performance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and researchers a streamlined sourcing pathway for IVD raw materials, including this antibody targeting MSR1, a central macrophage scavenger receptor implicated in atherosclerosis and innate immunity. Our supply capabilities encompass validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets, supporting your entire development workflow from concept to clinic.