Anti-Mouse CD19 Monoclonal Antibody () for Flow Cytometry - P25918

Core Product Specifications and Parameters

Biological Background: CD19 Function and Localization

• CD19 (B-lymphocyte antigen CD19, also known as differentiation antigen CD19) is a cell surface protein that functions as a coreceptor for the B-cell antigen receptor (BCR) complex on B lymphocytes.
• It lowers the threshold for BCR-mediated signaling, enhancing B-cell responses to antigens (By similarity).
• CD19 activates downstream signaling pathways including phosphatidylinositol 3-kinase (PI3K) and intracellular calcium mobilization.
  Related references: PMID:12387743 PMID:20101619 PMID:9382888
• Although not essential for early B-cell differentiation in the bone marrow, CD19 is required for normal B-1 cell development.
  Related references: PMID:12387743 PMID:7542548 PMID:7543183 PMID:9317126
• It is necessary for normal B-cell differentiation and proliferation in response to antigenic challenge.
  Related references: PMID:12387743 PMID:7542548 PMID:9317126
• CD19 contributes to humoral immunity by ensuring normal serum immunoglobulin levels and the production of high-affinity antibodies.
  Related references: PMID:12387743 PMID:7542548 PMID:7543183
• The protein is localized at the cell membrane and associates with membrane rafts.
• Tissue expression is predominantly in B cells of the spleen, bone marrow, thymus, lymph nodes, and peripheral blood.
  Related references: PMID:12387743 PMID:20101619 PMID:7542548 PMID:7543183
• CD19 is a glycoprotein and phosphoprotein that contains immunoglobulin-like domains, a transmembrane helix, and a cytoplasmic tail with signaling motifs.

Experimental Guidance and Technical Tips

• This antibody is directly conjugated to , enabling one-step staining for flow cytometry (FC). It is suitable for detecting CD19 expression on the surface of mouse B cells.
• Since the immunogen is recombinant full-length mouse CD19 protein, the antibody likely recognizes an extracellular epitope; confirm specificity by staining known CD19-positive and -negative cell lines or primary cells.
• For optimal performance, validate the antibody in the relevant sample system (e.g., mouse splenocytes, lymph node cells, or peripheral blood mononuclear cells) and titrate to determine the optimal concentration.
• Avoid prolonged exposure to light to preserve fluorophore stability, and consider using a viability dye to exclude dead cells during acquisition.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, laboratories, and research institutes a comprehensive, one-stop platform for IVD raw materials. This anti-mouse CD19 monoclonal antibody () is an example of our high-quality immune-reagent portfolio for B-cell biology and immuno-oncology research. Beyond individual antibodies, CamelBio provides validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to streamline your development pipeline.