Anti-Mouse CD161c/NK1.1 Monoclonal Antibody () for Flow Cytometry - P27814

Core Product Specifications and Parameters

Biological Background: CD161c/NK1.1 (Killer cell lectin-like receptor subfamily B member 1C) Function and Localization

• The target protein, killer cell lectin-like receptor subfamily B member 1C (Klrb1c), is also known as CD161c, NK1.1, NKR-P1C, and Ly-55c. It belongs to the killer cell lectin-like receptor (KLR) family and contains a C-type lectin domain.
• Klrb1c functions as a stimulatory receptor on natural killer (NK) cells, enhancing their cytotoxic activity. Cross-linking of the receptor triggers intracellular calcium mobilization and promotes interferon-gamma (IFN-γ) production, thereby amplifying the innate immune response.
• This receptor is a member of the NKR-P1 family, which includes both activating and inhibitory receptors that regulate NK cell function through recognition of specific ligands.
• Klrb1c is localized to the plasma membrane as a type II transmembrane protein, with its extracellular lectin domain oriented outward for ligand interaction and signaling.
• The protein is subject to post-translational modifications, including N-linked glycosylation and disulfide bond formation, which are critical for proper folding and stability.
• Expression of Klrb1c is primarily restricted to natural killer cells, making it a key marker for identifying and studying mouse NK cell populations in immunological research.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (amino acids 67–223) of mouse CD161c/NK1.1, which includes the ligand-binding region. This design supports specificity for native protein detection on cell surfaces.
• This conjugate is ready for direct flow cytometry; no secondary antibody is needed. Use appropriate controls (e.g., isotype control) and standard Fc-blocking steps to minimize background.
• Confirm antibody reactivity under your experimental conditions, as clone performance can vary with fixation and permeabilization protocols. For intracellular staining, validate permeabilization conditions to preserve epitope accessibility.
• Given the expression restricted to NK cells, ensure your sample preparation enriches for lymphocyte populations to obtain robust signal.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and researchers streamlined access to a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents. For projects targeting NK cell biology and immuno-oncology, we provide expertise in sourcing rare-target reagents like anti-CD161c/NK1.1 antibodies and complementary tools for multicolor flow cytometry panels. Partner with CamelBio to accelerate your assay development with reliable, high-quality raw-material supply.