Anti-Mouse CD150/SLAM Monoclonal Antibody for Flow Cytometry - Q9QUM4

Core Product Specifications and Parameters

Biological Background: CD150/SLAM Function and Localization

• CD150/SLAM (SLAMF1) is a self-ligand receptor of the signaling lymphocytic activation molecule family, which modulates the activation and differentiation of a wide variety of immune cells, integrating both innate and adaptive immunity.
• In T-cells, SLAMF1 mediates IL-2-independent proliferation, induces IFN-gamma production, and promotes TCR-induced IL-4 secretion by CD4+ cells; it is essential for IL-4 production by germinal center T follicular helper cells. Related references: PMID:12351401 PMID:9126961 PMID:15123745 PMID:20525889
• Its downstream signaling involves both SH2D1A/SAP-dependent and independent pathways, with divergent outcomes: the INPP5D-DOK1-DOK2 axis inhibits IFN-gamma, while PRKCQ-BCL10-NFKB1 promotes T-cell activation and Th2 cytokine production. Related references: PMID:11477403 PMID:15539155 PMID:16847311
• In conjunction with SLAMF6, controls the transition from positive selection to expansion and differentiation of thymic NKT cells, and drives peripheral iNKT cell differentiation toward a regulatory NKT2 phenotype. Related references: PMID:18031695 PMID:18606638
• In B-cells, SLAMF1 activates ERK and Akt signaling independently of SAP but dependent on SYK and INPP5D; together with CD84/SLAMF5 and SLAMF6, it may serve as a negative regulator of humoral immune responses. Related references: PMID:15315965 PMID:25926831
• In macrophages, SLAMF1 down-regulates IL-12, TNF, and nitric oxide in response to LPS, and mediates innate immune defense against Gram-negative bacteria by recognizing bacterial surface proteins (likely OmpC/OmpF), regulating phagosome maturation and PI3K complex II recruitment. Related references: PMID:15123745 PMID:20818396 PMID:22493499
• May also regulate allergen-induced Th2 and Th1 cytokine secretion, implicating a role in allergic diseases. Related references: PMID:16528012
• A transmembrane glycoprotein expressed on the cell membrane, consistent with its role in cell adhesion and signaling; it contains immunoglobulin-like domains and undergoes alternative splicing and phosphorylation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (aa 25-242) of mouse CD150/SLAM, indicating the antibody likely an extracellular epitope, making it suitable for detecting surface expression in live cells by flow cytometry.
• As a directly conjugated monoclonal antibody, it is optimized for flow cytometry; consider titrating to determine the optimal staining concentration for your specific cell population.
• Validate expression in relevant primary cells such as activated T cells, B cells, dendritic cells, macrophages, or NKT cells, where CD150/SLAM is known to be expressed.
• Include appropriate isotype controls and live/dead discrimination to ensure specific staining.
• Protect the antibody conjugate from light and avoid repeated freeze-thaw cycles to maintain fluorophore stability.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio accelerates your IVD development with a one-stop supply chain for high-performance raw materials, including validated antibody pairs and recombinant proteins. This CD150/SLAM monoclonal antibody exemplifies our commitment to providing optimized reagents for flow cytometry and immune cell profiling. From bulk ancillary reagents to custom sourcing for rare targets, we support your assay development from concept to clinic with streamlined logistics and technical expertise.