Anti-Mouse CD140b/PDGFR beta Monoclonal Antibody (PE) for Flow Cytometry - P05622

Core Product Specifications and Parameters

Biological Background: CD140b/PDGFR beta Function and Localization

• PDGFRB (also known as CD140b, PDGFR-1) is a receptor tyrosine kinase that binds platelet-derived growth factor (PDGF) ligands, including PDGFB, PDGFD, and PDGFA/PDGFB heterodimers, and is fundamental to embryonic development, cell proliferation, survival, differentiation, chemotaxis, and migration.
• The receptor plays a critical role in blood vessel development by promoting the proliferation, migration, and recruitment of pericytes and vascular smooth muscle cells to endothelial cells; it is essential for normal cardiovascular system formation and glomerular capillary branching in the kidney.
• PDGFRB belongs to the PDGF receptor family and can form homodimers or heterodimers with PDGFRA, with signaling outcomes modulated by ligand specificity and receptor dimer composition.
• Upon ligand binding, PDGFRB activates multiple downstream signaling cascades, including the PLCG1, PI3K-AKT, and RAS-MAPK pathways, leading to cytoskeletal rearrangements, membrane ruffling, and transcriptional responses.
• The receptor is predominantly located at the cell membrane, but also localizes to cytoplasmic vesicles and the lysosomal lumen following internalization, which contributes to signal attenuation.
• PDGFRB undergoes extensive post-translational modifications, including autophosphorylation on multiple tyrosine residues, N-linked glycosylation, and ubiquitination; it contains immunoglobulin-like domains, a kinase insert, and a split tyrosine kinase domain.
• In adult tissues, PDGFRB is weakly expressed in glomerular mesangial cells and interstitial cells, but its expression is markedly upregulated in renal fibrosis, suggesting a role in fibrotic disease progression.

Experimental Guidance and Technical Tips

• Since the immunogen corresponds to the extracellular domain (amino acids 32-530), the antibody likely recognizes the native surface-exposed receptor, making it suitable for flow cytometry on live, non-permeabilized cells.
• For flow cytometry, titrate the antibody to determine the optimal concentration for your specific cell type and staining conditions; consider using appropriate isotype controls and Fc receptor blocking to minimize non-specific binding.
• The antibody is validated for mouse; cross-reactivity with other species has not been tested; if using in other model systems, validate by comparing with known mouse PDGFRB expression.
• PDGFRB is a 123 kDa protein; ensure proper compensation when using PE-conjugated antibodies in multicolor flow panels, as PE is a bright fluorophore.
• Consider using enzymatic or non-enzymatic cell dissociation methods that preserve surface epitopes, as trypsinization may cleave the receptor and reduce staining intensity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, laboratories, and research institutes a comprehensive one-stop sourcing solution for IVD raw materials, technical services, and consulting, from concept to clinical application. This anti-CD140b/PDGFR beta monoclonal antibody supports cardiovascular development, fibrotic disease, and oncology research applications. Our portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom rare target raw-material sourcing to streamline your assay development.