Anti-Mouse CD130/gp130 Monoclonal Antibody for Flow Cytometry - Q00560

Core Product Specifications and Parameters

Biological Background: CD130/gp130 Function and Localization

• Interleukin-6 receptor subunit beta (IL6ST), commonly designated gp130 or CD130, functions as a shared signal-transducing subunit for multiple cytokine receptor complexes, including those for IL-6, IL-11, LIF, OSM, CNTF, CTF1, CLCF1, and other family members. Related references: PMID:1602143
• Upon ligand binding, gp130 homodimerizes (e.g., for IL-6/IL-11 receptors) or heterodimerizes with LIFR or OSMR (e.g., for LIF, CNTF), initiating downstream signaling. Related references: PMID:10661409, PMID:1602143
• Signaling proceeds through JAK/STAT, YAP, and NOTCH pathways, regulating immune responses, hematopoiesis, inflammation-induced epithelial regeneration, pain control, and bone metabolism. Related references: PMID:10661409, PMID:25731159, PMID:25057188, PMID:26255596
• Localized on the cell membrane, consistent with its role as a transmembrane receptor.
• Broadly expressed in tissues such as brain, heart, thymus, spleen, kidney, lung, and liver, and detected in many cell lines; also found in specific hypothalamic nuclei. Related references: PMID:28402851
• Post-translational modifications include N‑linked glycosylation and phosphorylation; the protein contains immunoglobulin-like domains, disulfide bonds, and a single transmembrane helix.
• Essential for embryonic development and for the survival of motor and sensory neurons, as well as astrocyte differentiation. Related references: PMID:10661409, PMID:10377352
• Required for normal trabecular bone mass and bone composition, and for osteoblast differentiation, partly via maintenance of PTH1R expression. Related references: PMID:25228504, PMID:9348227, PMID:24339143, PMID:26255596

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (aa 23-617), suggesting that this antibody likely recognizes an extracellular epitope; thus, live-cell staining for flow cytometry may be feasible without permeabilization.
• For flow cytometry, titrate the antibody on a gp130‑expressing mouse cell line or primary cells (e.g., splenocytes) to determine the optimal working concentration; always include an appropriate isotype control.
• Because gp130 is widely expressed, validate specificity by comparing staining between wild‑type and gp130‑knockout cells or by blocking with recombinant gp130 protein, if available.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, laboratories, and research institutes with a comprehensive portfolio of IVD raw materials and sourcing services. This anti‑mouse CD130/gp130 monoclonal antibody targets a pivotal cytokine receptor for immune, inflammatory, and bone metabolism studies, aligning with research needs in immunoassays and flow cytometry panels. Beyond individual antibodies, we supply validated antibody pairs, optimized monoclonal and polyclonal reagents, bulk ancillary reagents, and can assist in sourcing rare‑target raw materials—streamlining your development from concept to clinic.