Anti-Mouse CD130/gp130 Monoclonal Antibody for FC - Q00560

Core Product Specifications and Parameters

Biological Background: CD130/gp130 (IL6ST) Function and Localization

• CD130/gp130, encoded by the Il6st gene, is the interleukin-6 receptor subunit beta, also known as IL-6 signal transducer or membrane glycoprotein gp130 (UniProt entry Q00560).
• It functions as a shared signal-transducing receptor for multiple cytokines including IL-6, LIF, OSM, CNTF, IL-11, CT-1, and CLCF1. Upon cytokine binding, gp130 homodimerizes or heterodimerizes with LIFR or OSMR, activating JAK/STAT signaling pathways.
  Related references:
  PMID:1602143
  PMID:10661409
• The IL-6 signaling pathway induces expression of SOCS1 and SOCS3, which negatively regulate JAK activity as a feedback mechanism.
  Related references:
  PMID:9202125
• Gp130 also activates YAP and NOTCH pathways to control inflammation-induced epithelial regeneration, independent of STAT3.
  Related references:
  PMID:25731159
• It plays critical roles in immune response, hematopoiesis, pain modulation, bone metabolism, and embryonic development. It is essential for survival of motor and sensory neurons and astrocyte differentiation.
  Related references:
  PMID:10661409
  PMID:25057188
  PMID:26255596
  PMID:10377352
  PMID:25228504
  PMID:24339143
  PMID:9348227
• Subcellularly, gp130 is a single-pass type I membrane protein located at the cell membrane.
• It is widely expressed in various tissues including brain, heart, thymus, spleen, kidney, lung, and liver, and is found in many cell lines.
  Related references:
  PMID:28402851
• Post-translational modifications include disulfide bonds, glycosylation, and phosphorylation, and it contains immunoglobulin-like domains and fibronectin type III repeats.
• Required for expression of TRPA1 in nociceptive neurons and maintenance of PTH1R in osteoblasts, influencing pain sensation and bone formation.
  Related references:
  PMID:25057188
  PMID:25228504

Experimental Guidance and Technical Tips

• This -conjugated monoclonal antibody is designed for direct flow cytometry (FC) applications, eliminating the need for a secondary antibody.
• The immunogen corresponds to the extracellular domain (aa 23-617) of mouse CD130/gp130, so this antibody should recognize the native cell surface protein.
• Since gp130 is widely expressed, include appropriate positive and negative controls (e.g., knockout cells or isotype controls) to confirm specificity.
• For optimal staining, titrate the antibody in the relevant sample system and consider a viability dye to exclude dead cells.
• has excitation/emission maxima around 590/617 nm; verify compatibility with your flow cytometer's laser and filter configuration.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw-material sourcing partner, connecting diagnostic manufacturers, labs, and research institutes with high-quality antibodies and ancillary reagents. This anti-mouse CD130/gp130 monoclonal antibody supports research into cytokine signaling, inflammation, and immune regulation. Beyond this product, CamelBio can facilitate procurement of validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents, helping streamline your IVD development from concept to clinic.