Anti-Mouse CD127/IL-7Rα Monoclonal Antibody () for Flow Cytometry - P16872

Core Product Specifications and Parameters

Biological Background: Interleukin-7 receptor subunit alpha Function and Localization

• Interleukin-7 receptor subunit alpha (IL-7Rα, CD127) is a type I transmembrane glycoprotein that serves as the receptor for interleukin-7 (IL-7) and thymic stromal lymphopoietin (TSLP).
• It plays a critical role in lymphocyte development and homeostasis, mediating signaling pathways essential for T-cell and B-cell proliferation and survival.
• The receptor is a member of the hematopoietin receptor superfamily and forms heterodimers with the common gamma chain (γc) for IL-7 signaling or with TSLP receptor for TSLP signaling.
• Subcellular localization is at the plasma membrane, consistent with its function as a cell surface receptor.
• According to UniProt, mouse IL-7Rα is predominantly expressed in spleen, thymus, and fetal liver.
• The protein is post-translationally modified by glycosylation, disulfide bond formation, phosphorylation, and ubiquitination, which may regulate its stability and signaling activity.
• UniProt keywords include 3D-structure, Disulfide bond, Glycoprotein, Membrane, Phosphoprotein, Receptor, Reference proteome, Signal, Transmembrane, Transmembrane helix, and Ubl conjugation.

Experimental Guidance and Technical Tips

• The immunogen comprises the extracellular domain of mouse CD127/IL-7Rα (amino acids 21-239), suggesting the antibody likely recognizes an epitope in this region; verify epitope specificity in your system.
• This antibody is supplied as an conjugate and is validated for flow cytometry (FC); appropriate titers should be determined empirically.
• For optimal FC staining, consider using a recommended isotype control and proper Fc-blocking reagents to minimize non-specific binding.
• Cross-reactivity is reported only with mouse; if working with other species, validate reactivity independently.
• The predicted molecular weight of the target is approximately 51 kDa; this can be used as a reference in orthogonal validation assays but may vary due to glycosylation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and researchers with a comprehensive portfolio of validated antibody pairs, monoclonal/polyclonal antibodies, and ancillary reagents. For targets like CD127/IL-7Rα, critical in immune cell development, we offer one-stop access to raw materials from concept to clinic, including rare target sourcing and bulk supply.