Anti-Mouse CD127/IL-7Rα Monoclonal Antibody for Flow Cytometry - P16872

Core Product Specifications and Parameters

Biological Background: Interleukin-7 Receptor Subunit Alpha Function and Localization

• Interleukin-7 receptor subunit alpha (CD127/IL-7Rα) is a receptor that specifically binds interleukin-7 (IL-7), a cytokine critical for lymphocyte development and homeostasis.
• CD127 also functions as a receptor for thymic stromal lymphopoietin (TSLP), a cytokine involved in allergic inflammation and T cell regulation.
• The receptor is a single-pass type I membrane glycoprotein, localized on the cell surface membrane.
• It is primarily expressed in lymphoid tissues, including spleen, thymus, and fetal liver, consistent with its role in immune cell development.
• The protein contains disulfide bonds, is N-glycosylated, and undergoes phosphorylation and ubiquitination (Ubl conjugation), which may regulate receptor signaling and turnover.
• A 3D structure has been determined (per UniProt keyword), providing insights into ligand-receptor interaction mechanisms.

Experimental Guidance and Technical Tips

• The immunogen spans the extracellular domain (amino acids 21–239), making the antibody suitable for detecting surface-expressed CD127 by flow cytometry.
• For FC, consider titrating the antibody to determine optimal staining concentration and use an appropriate isotype control to set gating and compensation parameters.
• Verify antibody performance in your cell system of interest, and if performing intracellular staining, validate fixation and permeabilization conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers and research institutions to provide one-stop access to IVD raw materials, technical expertise, and supply chain solutions. This mouse CD127 monoclonal antibody supports immunology and immuno-oncology research; as part of our comprehensive portfolio, we offer validated antibody pairs, optimized recombinant proteins, bulk ancillary reagents, and proactive rare-target raw-material sourcing to accelerate your development from concept to clinic.