Anti-Mouse CD124/IL-4Ra Monoclonal Antibody for FC - P16382

Core Product Specifications and Parameters

Biological Background: Interleukin-4 Receptor Subunit Alpha Function and Localization

• The interleukin-4 receptor subunit alpha (IL-4Rα, CD124) is encoded by the Il4r gene in mouse and serves as a receptor subunit for both interleukin-4 (IL-4) and interleukin-13 (IL-13).
• IL-4Rα couples to the JAK1/2/3-STAT6 signaling pathway, playing a central role in promoting T helper 2 (Th2) cell differentiation.
• IL-4/IL-13 signaling via IL-4Rα regulates IgE production and stimulates chemokine and mucus production at sites of allergic inflammation.
• In certain cell types, IL-4Rα can also signal through activation of insulin receptor substrates (IRS1/IRS2), linking to metabolic pathways.
• The protein is located at the cell membrane and can also be secreted; it contains a transmembrane helix and is subject to alternative splicing.
• It is expressed in both Th1 and Th2 cells.
• Post-translational modifications include glycosylation, disulfide bond formation, and phosphorylation, consistent with its receptor function.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular region (amino acids 26-233), suggesting the antibody likely recognizes an epitope accessible on live cells, suitable for flow cytometry.
• For FC, consider titrating the antibody to determine the optimal concentration and include appropriate isotype controls to assess non-specific binding.
• Verify IL-4Rα expression on target cell populations; mouse Th2 cells and B cells are known to express the receptor.
• Avoid repeated freeze-thaw cycles; store according to manufacturer instructions (shipment on ice bags indicates short-term cold storage).

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a one-stop IVD raw-material partner, offering validated antibodies and ancillary reagents for immunoassay development. Our portfolio includes monoclonal and polyclonal antibodies targeting immune receptors like CD124/IL-4Rα, supporting allergic inflammation and Th2 differentiation research. Through our extensive sourcing network, we help diagnostic manufacturers secure rare target raw materials and accelerate projects from concept to clinic.