Anti-Mouse CD122 Monoclonal Antibody for Flow Cytometry - P16297

Core Product Specifications and Parameters

Biological Background: CD122 Function and Localization

• Interleukin-2 receptor subunit beta (IL-2RB), also known as CD122, p70, or high-affinity IL-2 receptor subunit beta, is encoded by the Il2rb gene in mice (UniProt P16297).
• Functions as the beta subunit of the receptor for interleukin-2 (IL-2), playing a critical role in receptor-mediated endocytosis and transduction of mitogenic signals.
• Likely associates with interleukin-15 receptor alpha (IL-15RA) to mediate the stimulation of neutrophil phagocytosis by IL-15 (by similarity).
• Localization: Cell membrane; single-pass type I membrane protein with an extracellular domain, a transmembrane helix, and a cytoplasmic tail.
• Post-translational modifications include disulfide bonds and glycosylation, contributing to its apparent molecular weight of ~60-75 kDa.
• Belongs to the type I cytokine receptor family; features a signal peptide, extracellular region, transmembrane domain, and cytoplasmic domain.
• Molecular weight: approximately 60.5 kDa (calculated from amino acid sequence).
• Keywords: 3D-structure, Cell membrane, Disulfide bond, Glycoprotein, Membrane, Receptor, Reference proteome, Signal, Transmembrane, Transmembrane helix.

Experimental Guidance and Technical Tips

• This antibody is directly conjugated to , eliminating the need for a secondary detection reagent in flow cytometry.
• As a rat monoclonal antibody targeting mouse CD122, ensure compatibility with your mouse sample and consider potential binding to Fc receptors.
• Titrate the antibody to determine the optimal working concentration for your specific cell type and staining conditions.
• Include appropriate isotype controls and viability dyes to ensure accurate gating and data interpretation.
• The immunogen is a synthetic peptide derived from mouse CD122; the epitope is linear and may be accessible in both live and fixed/permeabilized cells, but validation in your system is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, labs, and research institutes a streamlined pathway from concept to clinic, providing comprehensive access to IVD raw materials, technical services, and consulting. This anti-CD122 antibody supports immunology and inflammation research, and as your sourcing partner, we can supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and assist with rare target raw-material procurement—empowering your assay development and manufacturing needs.