Anti-Mouse CD11c Monoclonal Antibody () for Flow Cytometry - Q9QXH4

Core Product Specifications and Parameters

Biological Background: Integrin alpha-X Function and Localization

• Integrin alpha-X (CD11c) is a member of the integrin family, specifically pairing with beta-2 (CD18) to form the p150,95 complex.
• It functions as a receptor for fibrinogen, recognizing the G-P-R sequence, and facilitates cell-cell adhesion during inflammatory responses.
• This integrin plays a critical role in monocyte adhesion and chemotaxis, contributing to leukocyte trafficking and immune surveillance.
• Integrin alpha-X also acts as a receptor for erythrocyte-specific adhesion molecule ICAM4, mediating erythrophagocytosis by macrophages.
• Subcellularly, it is a transmembrane glycoprotein located on the cell membrane, with calcium- and magnesium-binding domains required for its adhesive functions.
• The protein contains multiple extracellular domains including repeats and a signal peptide, and is stabilized by disulfide bonds, characteristic of integrin alpha subunits.

Experimental Guidance and Technical Tips

• For flow cytometry, titrate the antibody to determine the optimal concentration for your specific cell population and staining conditions.
• Include appropriate isotype controls to assess non-specific binding, especially in complex mouse immune cell mixtures.
• When staining mouse samples, consider Fc receptor blocking to minimize background binding and improve resolution.
• Validate staining with a known CD11c-positive cell line or primary dendritic cells as a positive control.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive IVD raw-material sourcing for immunology and cell marker research, including validated antibody pairs and bulk reagents. This anti-Mouse CD11c antibody is part of our catalog supporting dendritic cell and myeloid lineage studies. Contact us for customized procurement and technical support across the research-to-clinic continuum.