anti-Mouse CD11c mAb for FC - Q9QXH4

Core Product Specifications and Parameters

Biological Background: CD11c Function and Localization

• Integrin alpha-X (CD11c) is a type I transmembrane glycoprotein that pairs with integrin beta-2 to form the p150,95 leukocyte adhesion receptor.
• Involved in cell-cell interactions during inflammatory responses, particularly mediating monocyte adhesion and chemotaxis.
• Recognizes the G-P-R sequence in fibrinogen and functions as a receptor for the erythrocyte adhesion molecule ICAM4, facilitating erythrophagocytosis.
• Belongs to the integrin alpha chain family, sharing structural features such as calcium-binding domains and disulfide-bonded regions.
• Expressed at the cell membrane, consistent with its role in adhesion and signaling.
• Contains disulfide bonds, is N-glycosylated, and features a signal peptide, transmembrane helix, and repeated domains characteristic of integrins.
• May contribute to dendritic cell and macrophage functions in immune surveillance and antigen presentation based on its cellular expression profile.

Experimental Guidance and Technical Tips

• This conjugate is designed for flow cytometry; direct immunofluorescence staining of mouse leukocytes is a typical application.
• Since the recombinant protein immunogen covers the mouse CD11c sequence, the antibody may recognize various epitopes; consider verifying specificity in knockout or knockdown models.
• For optimal staining, titrate the antibody to determine the appropriate working concentration for your cell type and experimental setup.
• As CD11c is expressed on dendritic cells and some macrophages/monocytes, include appropriate positive and negative controls to confirm staining patterns.
• The protein's molecular weight (~129 kDa) can serve as a reference for quality control; consider western blotting for orthogonal validation if the antibody is effective in denaturing conditions (note that FC-optimized reagents may not perform well in WB).

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