Anti-Mouse CD11b Monoclonal Antibody (PerCP) for Flow Cytometry - P05555

Core Product Specifications and Parameters

Biological Background: Integrin alpha-M (CD11b) Function and Localization

• Integrin alpha-M (CD11b) is the alpha subunit of the Mac-1 integrin, which pairs with the beta-2 subunit (ITGB2) to form a heterodimeric receptor expressed primarily on myeloid leukocytes.
• Mac-1 functions as a receptor for multiple ligands, including the iC3b fragment of complement component C3, fibrinogen, factor X, and ICAM1, mediating critical adhesive interactions of monocytes, macrophages, and granulocytes.
• It plays a key role in leukocyte adhesion, migration, and phagocytosis; specifically, it regulates neutrophil migration and may modulate phagocytosis-induced apoptosis in extravasated neutrophils.
• In the brain, CD11b is essential on microglia for TYROBP/DAP12-dependent production of superoxide ions, which influence neuronal apoptosis during development. Related references: PMID:18685038
• The protein localizes to the cell membrane and is enriched in membrane rafts, consistent with its role in signal transduction and adhesion.
• CD11b is predominantly expressed in monocytes and granulocytes, with additional expression in a subset of peritoneal mast cells and in microglia (at protein level).
• Keywords associated with CD11b include calcium binding, cell adhesion, glycoprotein, integrin, metal-binding, and receptor activity, reflecting its structural and functional characteristics.

Experimental Guidance and Technical Tips

• This antibody is a PerCP-conjugated rabbit monoclonal raised against a recombinant immunogen corresponding to amino acids 143-337 of mouse CD11b, which lies within the extracellular domain. Consider using it for flow cytometric analysis of mouse CD11b-expressing cells.
• For flow cytometry, appropriate controls (e.g., isotype control, live/dead discrimination, and Fc receptor blocking) should be included. Titration of the antibody in the specific experimental system is recommended to optimize signal-to-noise ratio.
• As the antibody is validated in mouse samples, cross-reactivity with other species is not expected; users should validate performance if applied to other species.
• The conjugate PerCP is compatible with standard flow cytometry instrumentation; avoid repeated freeze-thaw cycles and protect from prolonged light exposure to maintain conjugate stability.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive access to IVD raw materials and technical support for diagnostic manufacturers and research institutions. This anti-mouse CD11b antibody supports immunological research needs, complementing our range of validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. For rare target sourcing or custom requirements, CamelBio provides tailored procurement solutions to accelerate your project from concept to clinic.