Anti-Mouse CD11b Monoclonal Antibody () for Flow Cytometry - P05555

Core Product Specifications and Parameters

Biological Background: Integrin alpha-M (CD11b) Function and Localization

• Integrin alpha-M (CD11b, encoded by Itgam) is the alpha subunit of the ITGAM/ITGB2 (Mac-1/CR3) integrin complex and belongs to the integrin alpha chain family.
• It serves as a receptor for the iC3b fragment of complement component C3, mediating uptake of complement-coated particles and pathogens.
• The complex also binds fibrinogen, factor X, and ICAM1, facilitating leukocyte adhesion and neutrophil migration.
• In microglia, ITGAM/ITGB2 cooperates with TYROBP/DAP12 to regulate superoxide production, which promotes neuronal apoptosis during brain development. Related references: PMID:18685038
• Predominantly expressed in monocytes, granulocytes, and microglia, with specific expression in a subset of peritoneal mast cells. Related references: PMID:3887182 PMID:8986723 PMID:9862668 PMID:18685038
• Subcellular localization: cell membrane and membrane rafts.
• Features multiple disulfide bonds, glycosylation, and metal-binding (calcium) sites typical of integrins.

Experimental Guidance and Technical Tips

• For flow cytometry, titrate the antibody to determine the optimal working concentration for your specific cell type and experimental conditions.
• The conjugate is photolabile; protect from light during handling and storage.
• Validate the antibody in your sample system using appropriate controls, as cross-reactivity is confirmed only for mouse.
• Upon receipt, store at 2–8°C and protect from light; avoid freeze-thaw cycles.

CamelBio: Your One-Stop Sourcing Bridge

As a one-stop IVD raw-material sourcing partner, CamelBio provides this CD11b monoclonal antibody alongside a comprehensive portfolio of antibodies, antigens, and ancillary reagents for immunology and inflammation research. Our supply capabilities include validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk reagents, and rare-target raw-material sourcing, assisting your assay development from concept to clinic.