Anti-Mouse CD11b Monoclonal Antibody for Flow Cytometry - P05555

Core Product Specifications and Parameters

Biological Background: CD11b Function and Localization

• CD11b (Integrin alpha-M, Itgam) is encoded by the Itgam gene and is a transmembrane glycoprotein member of the integrin family. It partners with integrin beta-2 (CD18) to form the Mac-1 complex.
• It functions as a receptor for the iC3b fragment of complement (CR-3), fibrinogen, factor X, and ICAM1, mediating leukocyte adhesion and phagocytosis.
• ITGAM/ITGB2 is required for CD177-PRTN3-mediated activation of TNF-primed neutrophils and regulates neutrophil migration.
• In microglia, ITGAM works with TYROBP/DAP12 to produce superoxide ions that promote neuronal apoptosis during brain development (PubMed:18685038). Related references: PMID:18685038
• The protein is predominantly expressed in monocytes, granulocytes, a subset of peritoneal mast cells, and microglia.
• It localizes to the cell membrane and membrane rafts.
• Keywords indicate it has a signal peptide, undergoes alternative splicing, contains disulfide bonds, and is glycosylated. It also has calcium-binding and metal-binding sites.
• The molecular weight of the precursor is approximately 127 kDa.

Experimental Guidance and Technical Tips

• The immunogen corresponds to an extracellular region (amino acids 143–337) of mouse CD11b, suggesting the antibody recognizes the native protein on the cell surface.
• This product is specified for flow cytometry (FC). For other applications or species, consider performing appropriate validation in the relevant sample system.
• As the antibody is rabbit-derived and specific for mouse CD11b, ensure compatibility with secondary detection reagents and species-specific controls.
• The conjugate is suitable for multicolor flow cytometry panels; verify spectral compatibility with other fluorophores in your panel and instrument configuration.
• When using, include proper isotype controls and viability dyes to ensure accurate gating of monocyte, granulocyte, or microglia populations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supplies validated IVD raw materials for diagnostic manufacturers and research labs, including optimized monoclonal antibodies, antibody pairs, and ancillary reagents. For leukocyte adhesion or complement receptor studies, we can source rare-target reagents like this anti-CD11b mAb, along with technical consulting to streamline your development workflow. Our integrated supply chain helps you secure everything from bulk antibodies to specialized raw materials, bridging the gap from concept to clinic.